{"_buckets": {"deposit": "8593d2ab-b773-4d5e-9671-02bc6840274f"}, "_deposit": {"id": "21291", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "21291"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00021291", "sets": ["456", "1183"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Study on Molecular Structure, Expression and Degradation of Myelin-Associated Glycoprotein (MAG)"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1991-08", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "8", "bibliographicPageEnd": "547", "bibliographicPageStart": "540", "bibliographicVolumeNumber": "105", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "cDNA cloning of human myelin-associated glycoprotein (MAG) revealed complete amino acid sequence. In rodents, expression of the two forms of mRNA is developmentary regulated; the mRNA without exon 12 portion is expressed mainly in the active myelinating stage of development. Although the cDNA library used here was prepared from adult human brain poly (A)+ RNA all clones obtained corresponded to the mRNA without exon 12 portion. It has been demonstrated that the myelin membrane contains an endogenous calcium activated neutral protease (CANP) which degrades myelin basic protein (MBP) and MAG. The myelin endogenous CANP cleaves the 100KDa MAG molecule to a 90KDa derivative called derivative of MAG (dMAG). Since dMAG does not appear to be as tightly bound to membranes as intact MAG, the CANP is supposed (not proven) to cleave C-terminus of MAG. To confirm the proteolytic cleavage sites in MAG molecules, we raised antisera against the sequences of C-terminus of MAG. The peptides unique to large form of MAG (L-MAG) and small form of MAG (S-MAG) were synthesized using an automatic peptide synthesizer. We incubated purified myelin to produce dMAG and examined each anti-MAG antibody. Both anti-L-MAG and anti-S-MAG antibodies did not recognize dMAG. These results indicate that dMAG does not contain the sequences of C-termini in both L-MAG and S-MAG. Phosphorylation sites are thought to be important for functions of MAG as a recognition molecule and located near C-terminus of the cytoplasmic sequence. As dMAG lacks C-terminus, production of dMAG may result in the disturbance of myelin-axon interactions during demyelinating process.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "130356", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Sato, Shuzo"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "佐藤, 修三"}], "nameIdentifiers": [{"nameIdentifier": "130355", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-16"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "105(8)_540-547.pdf", "filesize": [{"value": "1.6 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1600000.0, "url": {"label": "105(8)_540-547.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/21291/files/105(8)_540-547.pdf"}, "version_id": "2e9cbd68-e0d1-4ebf-a802-3945746740fb"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "myelin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "myelin-associated glycoprotein", "subitem_subject_scheme": "Other"}, {"subitem_subject": "calcium activated neutral protease", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cDNA cloning", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ミエリン", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ミエリン糖蛋白(MAG)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "カルシウム依存性中性プロテアーゼ", "subitem_subject_scheme": "Other"}, {"subitem_subject": "cDNAクローニング", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "ヒトのmyelin-associated glycoprotein(MAG)の分子構造およびその発現と蛋白分解に関する研究", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "ヒトのmyelin-associated glycoprotein(MAG)の分子構造およびその発現と蛋白分解に関する研究"}, {"subitem_title": "ヒトのmyelin-associated glycoprotein(MAG)の分子構造およびその発現と蛋白分解に関する研究", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1183"], "permalink_uri": "http://hdl.handle.net/10191/39330", "pubdate": {"attribute_name": "公開日", "attribute_value": "2016-03-10"}, "publish_date": "2016-03-10", "publish_status": "0", "recid": "21291", "relation": {}, "relation_version_is_last": true, "title": ["ヒトのmyelin-associated glycoprotein(MAG)の分子構造およびその発現と蛋白分解に関する研究"], "weko_shared_id": null}