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ラット小脳におけるNSE, NNE mRNA発現の研究 : in situ hybridizationによる解析
http://hdl.handle.net/10191/40241
http://hdl.handle.net/10191/4024125085820-d71b-46a2-b7be-5cd27d34b90e
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
106(12)_1184-1192.pdf (3.2 MB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-03-25 | |||||
| タイトル | ||||||
| タイトル | ラット小脳におけるNSE, NNE mRNA発現の研究 : in situ hybridizationによる解析 | |||||
| タイトル | ||||||
| タイトル | ラット小脳におけるNSE, NNE mRNA発現の研究 : in situ hybridizationによる解析 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NSE | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | NNE | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | switch over | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | in situ hybridization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 神経特異的エノラーゼ | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | 神経非特異的エノラーゼ | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| その他のタイトル | ||||||
| その他のタイトル | A study on expression of NSE and NNE mRNA in rat cerebellum : in situ hybridization analysis | |||||
| 著者 |
片桐, 尚
× 片桐, 尚 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 126668 | |||||
| 姓名 | Katagiri, Takashi | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Using in situ hybridization technique, localization of mRNAs for neuron-specific enolase (NSE) and for non-neuronal enolase (NNE) was examined in the rat cerebellum at various postnatal developmental and adult stages. Probes used were synthetic oligonucleotides corresponding to 5' or 3' non-coding regions of rat NSE and NNE mRNA, respectively and were preliminarily confirmed to be specific by Northern blot hybridization. On examination of adult rat cerebellum, signals for NSE mRNA and for NNE mRNA were coinsidentally detected in the internal granule cells and Purkinje cells of the cerebellar cortex and in neurons of the dentate nucleus. In glial cells, only NNE signals were found. On examination of the cerebellum at postnatal developmental stages, coincidence of signals for NSE and NNE was observed in the internal granule cells, Purkinje cells and neurons of the dentate nucleus, weakly at postnatal day 0, more intensely at more developmental stages, and most intensely at abult stages. The external granule cells which were observed during postnatal day 0 to 10 revealed weak signals only for NNE mRNA. From these findings, it was shown that coincidental expression of NSE and NNE mRNA, both of which were more remarkable at more developmental stages, was common to various neurons in the rat cerebellum except for very undifferentiated external granule cells which revealed only weak NNE mRNA. | |||||
| 書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 106, 号 12, p. 1184-1192, 発行日 1992-12 |
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| 出版者 | ||||||
| 出版者 | 新潟医学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00290440 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00182415 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
片桐, 尚, 1992, ラット小脳におけるNSE, NNE mRNA発現の研究 : in situ hybridizationによる解析: 新潟医学会, 1184–1192 p.
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