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Time-Lapse Videomicrographic Observations of Parthenogenetic Mouse Embryos during Early Development
http://hdl.handle.net/10191/7771
http://hdl.handle.net/10191/77711ce6aa14-e3d9-48f6-9af3-7267d45f3b37
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2009030023.pdf (1.1 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
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| 公開日 | 2009-04-09 | |||||
| タイトル | ||||||
| タイトル | Time-Lapse Videomicrographic Observations of Parthenogenetic Mouse Embryos during Early Development | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Parthenogenetic mouse embryo | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Early development | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Time-lapse videomicrography | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | journal article | |||||
| 著者 |
Niimura, Sueo
× Niimura, Sueo× Futatsumata, Nana |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 6734 | |||||
| 姓名 | 新村, 末雄 | |||||
| 言語 | ja | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 6735 | |||||
| 姓名 | 二俣, ナナ | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The process of early development in diploid parthenogenetic mouse embryos from the 2-cell to the blastocyst stages was observed by time-lapse videomicrography, and was compared with that in fertilized embryos. Parthenogenetic 2-cell embryos cleaved and developed to 8-cell embryos after 23.0 hrs of culture. Transformation of blastomeres occurred at the 8-cell stage: namely, outer blastomeres were flattened. The embryos compacted at the morula stage, and then developed to blastocysts after 54.0 hrs of culture. A slit in the zona pellucida was formed 42.7 hrs after blastocyst formation, and trophectoderm cells protruded out of the zona pellucida through the slit. Protrusion of trophectoderm cells from the zona pellucida could arise from either side, polar trophectoderm or mural trophectoderm. After 7.0 hrs, the blastocysts completely escaped from the zona pellucida in either state, expansion or contraction. Fertilized 2-cell embryos showed morphological changes similar to those of parthenogenetic embryos and developed to blastocysts after 51.9 hrs of culture. Fertilized blastocysts took a significantly shorter time, 31.1 hrs, to start hatching, but required a significantly longer time, 23.8 hrs, to complete hatching, compared with parthenogenetic blastocysts. Hatching patterns of fertilized blastocysts were consistent with those of parthenogenetic blastocysts, except that hatching began with protrusion of trophectoderm cells from small holes in zonae pellucidae of fertilized blastocysts. From these results, it was confirmed that the developmental ability of early diploid parthenogenetic embryos prepared by the treatment with ethanol and cytochalasin B is comparable to that in fertilized embryos. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of mammalian ova research 巻 16, 号 3, p. 124-129, 発行日 1999 |
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| 出版者 | ||||||
| 出版者 | Japanese Society of Mammalian Ova Research | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-7738 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12018687 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1274/jmor.16.124 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
