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Contractions of Mouse Blastocysts Whose Hatching Abilities were Suppressed by Soybean Trypsin Inhibitor
http://hdl.handle.net/10191/7772
http://hdl.handle.net/10191/777254320cd1-c694-4bc2-9f2f-e7150e70b571
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
2009030024.pdf (1.6 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2009-04-09 | |||||
| タイトル | ||||||
| タイトル | Contractions of Mouse Blastocysts Whose Hatching Abilities were Suppressed by Soybean Trypsin Inhibitor | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Mouse | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Blastocyst hatching | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Contraction | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Soybean trypsin inhibitor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Time-lapse videomicrography | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Niimura, Sueo
× Niimura, Sueo× Wakasa, Ryoko |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 6730 | |||||
| 姓名 | 新村, 末雄 | |||||
| 言語 | ja | |||||
| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 6731 | |||||
| 姓名 | 若狭, 良子 | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | The role of contraction in blastocyst hatching was determined by time-lapse videomicrography in mouse blastocysts whose hatching ability had been suppressed by soybean trypsin inhibitor (STI). The hatching rate of blastocysts developed from morulae in a medium containing STI at a concentration of 1.0 mg/ml (STI-treated blastocysts) was 17.2%, which was significantly lower than the 63.9% for blastocysts developed from morulae in a medium without STI (non-treated blastocysts). Over the span of 32 hrs after blastocoel formation, the number of strong contractions was similar in STI-treated and nontreated blastocysts, but the total number of contractions and the number of weak contractions (less than 20% volume reduction) were significantly smaller in STI-treated blastocysts (4.22 and 2.94 times) than in non-treated blastocysts (5.80 and 4.50 times). These STI-treated blastocysts took a significantly longer time for weak contraction and for re-expansion after weak contraction (10.2 and 87.6 min), compared with non-treated blastocysts (7.8 and 58.2 min). It was also confirmed that the activity of trypsin-like proteinase was similar in STI-treated and non-treated blastocysts, and that a small hole was formed in the zona pellucida at the start of hatching in STI-treated blastocysts, as seen in non-treated blastocysts. Nevertheless, the rate of blastocysts with a slit resulting from enlargement of the small hole in the zona pellucida was 37.5% in STI-treated blastocysts, which was significantly lower than the 86.7% in non-treated blastocysts. These results suggest that weak contraction and re-expansion after weak contraction of blastocysts play an important role in hatching by slitting the region adjacent to the small hole in the zona pellucida. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Journal of mammalian ova research 巻 18, 号 1, p. 1-7, 発行日 2001 |
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| 出版者 | ||||||
| 出版者 | Japanese Society of Mammalian Ova Research | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1341-7738 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA12018687 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1274/jmor.18.1 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
