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PA/I38Tアミノ酸置換を有するバロキサビル感受性低下 : A型インフルエンザウイルスに対するサイクリングプローブリアルタイムPCR法の確立
http://hdl.handle.net/10191/0002000867
http://hdl.handle.net/10191/0002000867aac4856a-3fef-4fa8-bb4f-74a8917980cd
名前 / ファイル | ライセンス | アクション |
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本文 (688KB)
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要旨 (556KB)
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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公開日 | 2023-03-07 | |||||||||
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言語 | en | |||||||||
タイトル | Development of cycling probe based real-time PCR methodology for influenza A viruses possessing the PA/I38T amino acid substitution associated with reduced baloxavir susceptibility | |||||||||
タイトル | ||||||||||
言語 | ja | |||||||||
タイトル | PA/I38Tアミノ酸置換を有するバロキサビル感受性低下 : A型インフルエンザウイルスに対するサイクリングプローブリアルタイムPCR法の確立 | |||||||||
言語 | ||||||||||
言語 | eng | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Influenza virus | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Baloxavir marboxil | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | PA/I38T substitution | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Cycling probe real-time PCR | |||||||||
キーワード | ||||||||||
言語 | en | |||||||||
主題Scheme | Other | |||||||||
主題 | Antiviral susceptibility | |||||||||
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資源 | http://purl.org/coar/resource_type/c_db06 | |||||||||
タイプ | doctoral thesis | |||||||||
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アクセス権 | open access | |||||||||
アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
著者 |
長田, 秀和
× 長田, 秀和
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抄録 | ||||||||||
内容記述タイプ | Abstract | |||||||||
内容記述 | Baloxavir marboxil has been used for influenza treatment since March 2018 in Japan. After baloxavir treatment, the most frequently detected substitution is Ile38Thr in polymerase acidic protein (PA/I38T), and this substitution reduces baloxavir susceptibility in influenza A viruses. To rapidly investigate the frequency of PA/I38T in influenza A (H1N1)pdm09 and A (H3N2) viruses in clinical samples, we established a rapid real-time system to detect single nucleotide polymorphisms in PA, using cycling probe real-time PCR. We designed two sets of probes that were labeled with either 6-carboxyfluorescein (FAM) or 6-carboxy-X-rhodamine (ROX) to identify PA/I38(wild type strain) or PA/I38T, respectively. The established cycling probe real-time PCR system showed a dynamic linear range of 101 to 106 copies with high sensitivity in plasmid DNA controls. This real-time PCR system discriminated between PA/I38T and wild type viruses well. During the 2018/19 season, 377 influenza A-positive clinical samples were collected in Japan before antiviral treatment. Using our cycling probe real-time PCR system, we detected no (0/129, 0.0%) influenza A (H1N1)pdm09 viruses with PA/I38T substitutions and four A(H3N2) (4/229, 1.7%) with PA/I38T substitution prior to treatment. In addition, we found PA/I38T variant in siblings who did not received baloxavir treatment during an infection caused by A (H3N2) that afflicted the entire family. Although human-to-human transmission of PA/I38T variant may have occurred in a closed environment, the prevalence of this variant in influenza A viruses was still limited. Our cycling probe-PCR system is thus useful for antiviral surveillance of influenza A viruses possessing PA/I38T. | |||||||||
言語 | en | |||||||||
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内容記述タイプ | Other | |||||||||
内容記述 | Antiviral research. 2021, 188, 105036. | |||||||||
言語 | en | |||||||||
DOI | ||||||||||
識別子タイプ | DOI | |||||||||
関連識別子 | https://doi.org/10.1016/j.antiviral.2021.105036 | |||||||||
権利 | ||||||||||
言語 | en | |||||||||
権利情報 | © 2021 Elsevier B.V. All rights reserved. | |||||||||
学位名 | ||||||||||
言語 | ja | |||||||||
学位名 | 博士(医学) | |||||||||
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学位授与機関識別子Scheme | kakenhi | |||||||||
学位授与機関識別子 | 13101 | |||||||||
言語 | ja | |||||||||
学位授与機関名 | 新潟大学 | |||||||||
言語 | en | |||||||||
学位授与機関名 | Niigata University | |||||||||
学位授与年月日 | ||||||||||
学位授与年月日 | 2022-03-23 | |||||||||
学位授与番号 | ||||||||||
学位授与番号 | 甲第5011号 | |||||||||
学位記番号 | ||||||||||
内容記述タイプ | Other | |||||||||
内容記述 | 新大院博(医)第1081号 | |||||||||
言語 | ja |