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  1. 260 大学院医歯学総合研究科(歯)
  2. 20 紀要
  3. 01 新潟歯学会雑誌
  4. 第43巻第2号
  1. 0 資料タイプ別
  2. 03 紀要論文

口腔粘膜上皮細胞培養上清で培養した口腔粘膜線維芽細胞の形質に関する検討

http://hdl.handle.net/10191/0002000494
http://hdl.handle.net/10191/0002000494
1616cd4b-e7b4-4473-a5aa-090844773f48
名前 / ファイル ライセンス アクション
43(2)_105-112.pdf 43(2)_105-112.pdf (1.14MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2022-05-25
タイトル
言語 ja
タイトル 口腔粘膜上皮細胞培養上清で培養した口腔粘膜線維芽細胞の形質に関する検討
タイトル
言語 en
タイトル Characterization of an oral fibroblast phenotype cultured in oral keratinocyte-conditioned medium
言語
言語 eng
キーワード
言語 ja
主題Scheme Other
主題 口腔粘膜線維芽細胞
キーワード
言語 ja
主題Scheme Other
主題 口腔粘膜角化細胞
キーワード
言語 ja
主題Scheme Other
主題 培養上清
キーワード
言語 ja
主題Scheme Other
主題 形質
キーワード
言語 ja
主題Scheme Other
主題 単層培養
キーワード
言語 en
主題Scheme Other
主題 Oral fibroblast
キーワード
言語 en
主題Scheme Other
主題 oral keratinocyte
キーワード
言語 en
主題Scheme Other
主題 conditioned medium
キーワード
言語 en
主題Scheme Other
主題 phenotype
キーワード
言語 en
主題Scheme Other
主題 monolayer culture
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
アクセス権
アクセス権 open access
アクセス権URI http://purl.org/coar/access_right/c_abf2
著者 モニル, マザビンビンタ

× モニル, マザビンビンタ

ja モニル, マザビンビンタ

en Monir, Mah Zabin Binta

Search repository
抄録
内容記述タイプ Abstract
内容記述 Fibroblasts in monoculture grown in serum-containing medium are highly-proliferating. However, data suggested that use of static fibroblasts provides a better model to study biological phenomena than proliferating fibroblasts. Since previous studies have stated keratinocyte-conditioned medium reduced fibroblast proliferation, this study aimed to examine if the oral keratinocyte (OK) -conditioned medium (CM) can decrease oral fibroblasts (OF) proliferation and to characterize their phenotype. Primary human OK and OF were grown in a completed EpiLife^<【○!R】>(0.06mM Ca^<++>) and Dulbecco's modified Eagle medium (DMEM) containing 10% calf serum (DMEM-CS), respectively. OK-CM was conditioned for 24 hours in a near-confluent OK culture. OFs plated in a micro-plate well were cultured with DMEM-CS, serum-free DMEM (SF-DMEM) and OK-CM for up to 96 hours. Proliferation rate and cell cycle profile were analyzed using a MTT assay and a fluorescence-activated cell sorter. The "phenotypic changes" of OFs were determined by the activity of senescent-associated β-galactosidase (β-gal) and the secreted protein levels including keratinocyte growth factor (KGF), human type I collagen and matrix metalloproteinase-1 (MMP-1) measured by enzyme-linked immunosorbent assay (ELISA). The proliferating rate and the proportion of cells in S phase were significantly lower when cells were cultured in OK-CM. The β-gal activity suggested OFs in OK-CM still had proliferating potential. ELISA assay showed OFs cultured in OK-CM produce KGF and MMP-1 as did OFs grown in DMEM-CS while their ability to produce type I collagen was significantly lower than OFs in DMEM-CS. This study suggested the OK-CM generated a quiescent OF population possessing the characteristic of extracellular matrix degradation rather than synthesis.
言語 en
抄録
内容記述タイプ Abstract
内容記述 生体反応の研究に用いる線維芽細胞は増殖している細胞ではなく休止期のものが好ましい。上皮細胞の培養上清に線維芽細胞の増殖抑制効果があることが報告されている。本研究の目的は口腔粘膜上皮細胞の培養上清が培養口腔粘膜線維芽細胞の増殖性を抑え,細胞形質を変化させるかを検証することである。ヒト初代口腔粘膜上皮細胞(OK)と線維芽細胞(OF)はそれぞれEpiLifeと10%ウシ胎児血清含有ダルベッコ改変イーグル培地(DMEM)(DMEM-CS)中で培養した。コンフルエントに近い口腔粘膜上皮細胞を24時間培養したものを上清とした。口腔粘膜線維芽細胞はDMEM-CS,DMEM培地単独(SF-DMEM),口腔粘膜上皮細胞培養上清(OK-CM)の3種類の培地で,最長96時間培養した。細胞増殖能と細胞周期はそれぞれMTT assayとセルソーターで解析した。細胞形質変化の分析はさらに,βガラクトシダーゼ活性発現とエライザ法(ELISA)による各種タンパク質(ケラチノサイト増殖因子(KGF),タイプIコラーゲン,マトリックスメタロプロテアーゼ-1(MMP-1))分泌量で検討した。細胞増殖能とS期の細胞の割合は有意にOK-CMで培養した細胞で低下しており,G0/G1期停止による細胞増殖抑制の可能性が示唆された。またOK-CM培養では,βガラクトシダーゼ活性を示した細胞は少数で,口腔粘膜線維芽細胞は休止期にいるが,増殖能が失われていないことを示した。KGFとMMP-1の産生量は,DMEM-CSで培養した細胞と同等であったのに対し,I型コラーゲンの産生量が有意に低かった。本研究から,OK-CMは培養口腔粘膜線維芽細胞の増殖能を抑え,かつ細胞外基質分解性の細胞形質となることが示唆された。
言語 ja
書誌情報 ja : 新潟歯学会雑誌

巻 43, 号 2, p. 105-112, 発行日 2013-12
出版者
言語 ja
出版者 新潟歯学会
ISSN
収録物識別子タイプ PISSN
収録物識別子 0385-0153
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
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