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シンビンディンの発現低下がスリット膜の機能障害に関与している
http://hdl.handle.net/10191/0002000473
http://hdl.handle.net/10191/00020004738e4cb639-3fca-4d3a-9bf0-3f8230fd7d9d
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本文 (1.16MB)
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要旨 (439KB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||||||
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| 公開日 | 2022-05-10 | |||||||||
| タイトル | ||||||||||
| タイトル | Synbindin downregulation participates in slit diaphragm dysfunction | |||||||||
| 言語 | en | |||||||||
| タイトル | ||||||||||
| タイトル | シンビンディンの発現低下がスリット膜の機能障害に関与している | |||||||||
| 言語 | ja | |||||||||
| 言語 | ||||||||||
| 言語 | eng | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | synbindin | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | podocyte | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | slit diaphragm | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | nephrin | |||||||||
| キーワード | ||||||||||
| 言語 | en | |||||||||
| 主題Scheme | Other | |||||||||
| 主題 | SV2B | |||||||||
| 資源タイプ | ||||||||||
| 資源 | http://purl.org/coar/resource_type/c_db06 | |||||||||
| タイプ | doctoral thesis | |||||||||
| アクセス権 | ||||||||||
| アクセス権 | open access | |||||||||
| アクセス権URI | http://purl.org/coar/access_right/c_abf2 | |||||||||
| その他のタイトル | ||||||||||
| その他のタイトル | Synbindin in podocytes | |||||||||
| 言語 | en | |||||||||
| 著者 |
イワノフ, ベニアミン
× イワノフ, ベニアミン
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| 抄録 | ||||||||||
| 内容記述タイプ | Abstract | |||||||||
| 内容記述 | Introduction: Synbindin, originally identified as a neuronal cytoplasmic molecule, was found in glomeruli. cDNA subtractive hybridization technique showed the mRNA expression of synbindin in glomeruli was down-regulated in puromycin aminonucleoside (PAN) nephropathy, a mimic of minimal-change nephrotic syndrome. Methods: The expression of synbindin in podocyte was analyzed in normal rats and two types of rat nephrotic models, anti-nephrin antibody (ANA)-induced nephropathy a pure slit diaphragm injury model and PAN nephropathy by immunohistochemical analysis and RT-PCR techniques. To elucidate the function of synbindin, a gene silencing study with human cultured podocytes was performed. Results: Synbindin was mainly expressed at slit diaphragm area of glomerular epithelial cells (podocytes). In both nephrotic models, decreased mRNA expression and the altered staining of synbindin were already detected at the early phase when proteinuria and the altered staining of nephrin, a key molecule of slit diaphragm, were not detected yet. Synbindin staining was clearly reduced when severe proteinuria was observed. When the cultured podocytes were treated with siRNA for synbindin, the cell changed to be round-shape, and filamentous actin structure was clearly altered. The expression of ephrin-B1, a transmembrane protein at slit diaphragm, was clearly lowered, and synaptic vesicle-associated protein 2B (SV2B) was upregulated in the synbindin knockdown cells. Conclusion: Synbindin participates in maintaining foot processes and slit diaphragm as a downstream molecule of SV2B-mediated vesicle transport. Synbindin downregulation participates in slit diaphragm dysfunction. Synbindin can be an early marker to detect podocyte injury. | |||||||||
| 言語 | en | |||||||||
| 内容記述 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | American journal of nephrology. 2021, 52(8), 620-629. | |||||||||
| 言語 | en | |||||||||
| DOI | ||||||||||
| 識別子タイプ | DOI | |||||||||
| 関連識別子 | https://doi.org/10.1159/000517975 | |||||||||
| 権利 | ||||||||||
| 言語 | en | |||||||||
| 権利情報 | This is the accepted manuscript version of an article published by S. Karger AG in [American journal of nephrology/2021/52/8/620-629] including https://doi.org/10.1159/000517975 and available on https://www.karger.com/Article/FullText/000517975. The final, published version of this article is available at https://www.karger.com/?doi=10.1159/000517975. | |||||||||
| 学位名 | ||||||||||
| 言語 | ja | |||||||||
| 学位名 | 博士（医学） | |||||||||
| 学位授与機関 | ||||||||||
| 学位授与機関識別子Scheme | kakenhi | |||||||||
| 学位授与機関識別子 | 13101 | |||||||||
| 言語 | ja | |||||||||
| 学位授与機関名 | 新潟大学 | |||||||||
| 言語 | en | |||||||||
| 学位授与機関名 | Niigata University | |||||||||
| 学位授与年月日 | ||||||||||
| 学位授与年月日 | 2021-09-21 | |||||||||
| 学位授与番号 | ||||||||||
| 学位授与番号 | 甲第4941号 | |||||||||
| 学位記番号 | ||||||||||
| 内容記述タイプ | Other | |||||||||
| 内容記述 | 新大院博(医)第1031号 | |||||||||
| 言語 | ja | |||||||||
