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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 230 大学院自然科学研究科
  2. 10 学術雑誌論文
  3. 10 査読済論文

Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses

http://hdl.handle.net/10191/24665
http://hdl.handle.net/10191/24665
20be9627-0df6-4bc3-b922-4225d1b7ac30
名前 / ファイル ライセンス アクション
110006203475.pdf 110006203475.pdf (843.8 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-01-08
タイトル
言語 en
タイトル Early Identification of Intra- and Intergeneric Hybrids among Colchicaceous Ornamentals, Gloriosa spp., Littonia modesta Hook. and Sandersonia aurantiaca Hook., by Flow Cytometry and Random Amplified Polymorphic DNA Analyses
言語
言語 eng
キーワード
主題Scheme Other
主題 colchicaceous ornamentals
キーワード
主題Scheme Other
主題 flow cytometry
キーワード
主題Scheme Other
主題 hybrid identification
キーワード
主題Scheme Other
主題 RAPD
キーワード
主題Scheme Other
主題 wide hybridization
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
その他のタイトル
その他のタイトル フローサイトメトリー分析および RAPD 分析によるコルチカム科花き園芸植物(グロリオーサ属植物,リットニアおよびサンダーソニア)における属内および属間雑種の早期同定
言語 ja
著者 Amano, Junji

× Amano, Junji

WEKO 6481

en Amano, Junji

Search repository
Kuwayama, Sachiko

× Kuwayama, Sachiko

WEKO 6482

en Kuwayama, Sachiko

Search repository
Mizuta, Yoko

× Mizuta, Yoko

WEKO 6483

en Mizuta, Yoko

Search repository
Oomiya, Tomo

× Oomiya, Tomo

WEKO 6484

en Oomiya, Tomo

Search repository
Nakamura, Toru

× Nakamura, Toru

WEKO 6485

en Nakamura, Toru

Search repository
Nakano, Masaru

× Nakano, Masaru

WEKO 6486

en Nakano, Masaru

Search repository
抄録
内容記述タイプ Abstract
内容記述 Plantlets derived from ovule culture following intra- or intergeneric crosses among Colchicaceous ornamentals, Gloriosa spp., Littonia modesta Hook, and Sandersonia aurantiaca Hook., were subjected to flow cytometry (FCM) and random amplified polymorphic DNA (RAPD) analyses in order to verify their hybridity. For crosses between plants with apparently distinct relative fluorescence intensity (RFI) of nuclei, i.e., intrageneric crosses between Gloriosa genotypes with different ploidy levels, and most intergeneric crosses, detection of hybrid plantlets could be readily accomplished by FCM analysis. The results of hybrid identification by RAPD analysis supported those of FCM analysis. In addition, RAPD analysis allowed the verification of the hybridity of intra- or intergeneric cross-derived plantlets, which could not be identified as hybrids by FCM analysis due to the similarity of RFI of the parents or appearance of the RFI peak in an unexpected position. Totally, 110 independent hybrid plantlets (60 intrageneric and 50 intergeneric hybrids) have so far been identified by FCM and/or RAPD analyses. Thus, FCM in combination with RAPD analyses offer simple and rapid means for the early detection of intrageneric and intergeneric hybrids in Colchicaceous ornamentals.
言語 en
書誌情報 en : Journal of the Japanese Society for Horticultural Science

巻 76, 号 1, p. 73-78, 発行日 2007-01
出版者
言語 ja
出版者 園芸学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 1882-3351
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12177046
DOI
識別子タイプ DOI
関連識別子 https://doi.org/10.2503/jjshs.76.73
権利
権利情報 園芸学会
権利
権利情報 本文データは学協会の許諾に基づきCiNiiから複製したものである
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
異版である
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 http://ci.nii.ac.jp/naid/110006203475
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