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細胞生物学や病理学におけるmRNAの検出法とその応用
http://hdl.handle.net/10191/37894
http://hdl.handle.net/10191/37894ef43cc65-2fa7-4ed7-b5b8-9389f4babbc5
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
108(1)_1-5.pdf (810.0 kB)
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| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2016-02-08 | |||||
| タイトル | ||||||
| タイトル | 細胞生物学や病理学におけるmRNAの検出法とその応用 | |||||
| タイトル | ||||||
| タイトル | 細胞生物学や病理学におけるmRNAの検出法とその応用 | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | jpn | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | mRNA | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Ribonuclease protection assay | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | In situ hybridization | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | メッセンジャー RNA | |||||
| 資源タイプ | ||||||
| 資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
| 資源タイプ | departmental bulletin paper | |||||
| その他のタイトル | ||||||
| その他のタイトル | Methods for mRNA Detection and Their Application in Cell Biology and Pathology | |||||
| 著者 |
山本, 格
× 山本, 格 |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 121660 | |||||
| 姓名 | Yamamoto, Tadashi | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | By molecular biology new important proteins and peptides have been cloned. Detection, localization and quantitation of these proteines and peptides in cells, tissues or organs are aimed in cell biology or pathology. Immunological techniques using antibodies as probes have been employed for this purpose, however, antibodies against the newly-discovered substances are often unavailable. To solve the problem, methods to detect mRNA using nucleic acids as probes are recently devised. This review introduces two representative techniques to detect mRNA in total cellular RNA extracted from cell or tissue samples and to localize mRNA in tissue sections. Ribonuclease (RNase) protection assay is more simple, sensitive and quantitative than Northern blot analysis for detection of mRNA in total cellular RNA samples. In addition, expression of several different mRNAs can be examined in a single sample by this method using multiple probes. In situ hybridization is a method to localize mRNA in cells in tissue sections. As their applications presented are expression of mRNA for cytokines and growth factors in glomeruli of rats with experimental glomerulonephritis and localization of mRNA for mRNA for type A atrial natriuretic peptide receptor in glomeruli. | |||||
| 書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 108, 号 1, p. 1-5, 発行日 1994-01 |
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| 出版者 | ||||||
| 出版者 | 新潟医学会 | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 00290440 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AN00182415 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
