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Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins
http://hdl.handle.net/10191/24668
http://hdl.handle.net/10191/24668e8f3d63c-ecce-4f75-b6dc-b6c939f73e77
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
110001808113.pdf (1.0 MB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2014-01-08 | |||||
| タイトル | ||||||
| タイトル | Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | Hemerocallis hybrida | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | DNA synthesis inhibitor | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | micronucleation | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | metaphase index(MI) | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | spindle toxin | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Saito, Hiroyuki
× Saito, Hiroyuki× Nakano, Masaru |
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| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | For the production of intergeneric hybrid plants with partial alien genome via microprotoplast fusion in the Liliaceous ornamentals, the effects of the DNA synthesis inhibitor, hydroxyurea(HU), and the spindle toxins, colchicine(COL), oryzalin(ORY), amiprophos-methyl(APM), butamiphos(BUT), isopropyl N-(3-chlorophenyl)carbamate(CIPC)and propyzamide(PRO)on the metaphase index(MI)and the percentage of micronucleated cells(micronucleus index ; MNI)were examined in cell suspension cultures of Hemerocallis hybrida cv.Stella d'Oro. Suspension cells were subcultured every three days in MS medium containing 10 mgl^<-1> picloram. Although MI was only 2-3% in the asynchronous control cultures, it increased up to 8.9% and 9.7% by treatment of the cultures with HU and COL, respectively. In addition, MI was further increased by using the sequential treatments of the cultures with HU and each spindle toxin:the highest MI of 30.5% was obtained by treatment with 2mM HU for 24h followed by that with 250 μM COL for 20h. COL and ORY were more effective for synchronizing cell division than the other four spindle toxins. The effects of various spindle toxin treatments on the micronucleation of suspension cultures were also examined by combining the HU pre-treatment for 24h. Among the six spindle toxins, COL and ORY induced few micronuclei, whereas APM, BUT, CIPC and PRO induced micronucleation in cells to various extents. The most effective treatment for micronucleation was that with 8 μM PRO for 66h, where MNI was 14.7% and the number of micronuclei per cell ranged from 1-7 were obtained. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Breeding science 巻 51, 号 4, p. 285-291, 発行日 2001-12 |
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| 出版者 | ||||||
| 出版者 | 日本育種学会 | |||||
| 言語 | ja | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 1344-7610 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA11353132 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1270/jsbbs.51.285 | |||||
| 権利 | ||||||
| 権利情報 | 日本育種学会 | |||||
| 権利 | ||||||
| 権利情報 | 本文データは学協会の許諾に基づきCiNiiから複製したものである | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
| 異版である | ||||||
| 関連タイプ | isVersionOf | |||||
| 識別子タイプ | URI | |||||
| 関連識別子 | http://ci.nii.ac.jp/naid/110001808113 | |||||
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Cite as
Saito, Hiroyuki, Nakano, Masaru, 2001, Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins: 日本育種学会, 285–291 p.
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