{"_buckets": {"deposit": "c844de4e-c337-426d-9140-d03cdecd70ad"}, "_deposit": {"id": "1595", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1595"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001595", "sets": ["454", "470"]}, "author_link": ["4502", "4503"], "control_number": "1595", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2001-12", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "4", "bibliographicPageEnd": "291", "bibliographicPageStart": "285", "bibliographicVolumeNumber": "51", "bibliographic_titles": [{"bibliographic_title": "Breeding science", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "For the production of intergeneric hybrid plants with partial alien genome via microprotoplast fusion in the Liliaceous ornamentals, the effects of the DNA synthesis inhibitor, hydroxyurea(HU), and the spindle toxins, colchicine(COL), oryzalin(ORY), amiprophos-methyl(APM), butamiphos(BUT), isopropyl N-(3-chlorophenyl)carbamate(CIPC)and propyzamide(PRO)on the metaphase index(MI)and the percentage of micronucleated cells(micronucleus index ; MNI)were examined in cell suspension cultures of Hemerocallis hybrida cv.Stella d\u0027Oro. Suspension cells were subcultured every three days in MS medium containing 10 mgl^\u003c-1\u003e picloram. Although MI was only 2-3% in the asynchronous control cultures, it increased up to 8.9% and 9.7% by treatment of the cultures with HU and COL, respectively. In addition, MI was further increased by using the sequential treatments of the cultures with HU and each spindle toxin:the highest MI of 30.5% was obtained by treatment with 2mM HU for 24h followed by that with 250 μM COL for 20h. COL and ORY were more effective for synchronizing cell division than the other four spindle toxins. The effects of various spindle toxin treatments on the micronucleation of suspension cultures were also examined by combining the HU pre-treatment for 24h. Among the six spindle toxins, COL and ORY induced few micronuclei, whereas APM, BUT, CIPC and PRO induced micronucleation in cells to various extents. The most effective treatment for micronucleation was that with 8 μM PRO for 66h, where MNI was 14.7% and the number of micronuclei per cell ranged from 1-7 were obtained.", "subitem_description_language": "en", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "日本育種学会", "subitem_publisher_language": "ja"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.1270/jsbbs.51.285", "subitem_relation_type_select": "DOI"}}]}, "item_5_relation_31": {"attribute_name": "異版である", "attribute_value_mlt": [{"subitem_relation_type": "isVersionOf", "subitem_relation_type_id": {"subitem_relation_type_id_text": "http://ci.nii.ac.jp/naid/110001808113", "subitem_relation_type_select": "URI"}}]}, "item_5_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "日本育種学会"}, {"subitem_rights": "本文データは学協会の許諾に基づきCiNiiから複製したものである"}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AA11353132", "subitem_source_identifier_type": "NCID"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1344-7610", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Saito, Hiroyuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4502", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Nakano, Masaru", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4503", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "110001808113.pdf", "filesize": [{"value": "1.0 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1000000.0, "url": {"label": "110001808113.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1595/files/110001808113.pdf"}, "version_id": "b1af0067-2495-4604-865b-a4b57c081dbe"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Hemerocallis hybrida", "subitem_subject_scheme": "Other"}, {"subitem_subject": "DNA synthesis inhibitor", "subitem_subject_scheme": "Other"}, {"subitem_subject": "micronucleation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "metaphase index(MI)", "subitem_subject_scheme": "Other"}, {"subitem_subject": "spindle toxin", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "470"], "permalink_uri": "http://hdl.handle.net/10191/24668", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-01-08"}, "publish_date": "2014-01-08", "publish_status": "0", "recid": "1595", "relation": {}, "relation_version_is_last": true, "title": ["Partial Synchronization of Cell Division and Micronucleation in Suspension-cultured Cells of Hemerocallis hybrida : The effects of Hydroxyurea and Various Spindle Toxins"], "weko_shared_id": -1}