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Chitin binding protein (CBP21) in the culture supernatant of Serratia marcescens 2170
http://hdl.handle.net/10191/6331
http://hdl.handle.net/10191/63319ec4804c-58f7-4b1a-b038-5e5557e1a0c0
名前 / ファイル | ライセンス | アクション |
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07_0007.pdf (1.4 MB)
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-23 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Chitin binding protein (CBP21) in the culture supernatant of Serratia marcescens 2170 | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chitin binding protein | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Serratia marcescens | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | chitinase | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | nucleotide sequence | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | journal article | |||||
著者 |
Suzuki, Kazushi
× Suzuki, Kazushi× Suzuki, Megumi× Taiyoji, Mayumi× Nikaidou, Naoki× Watanabe, Takeshi |
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著者別名 | ||||||
識別子 | 4401 | |||||
識別子Scheme | WEKO | |||||
姓名 | 鈴木, 一史 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | A chitin binding protein (CBP21) 21 kDa in size, is a major protein in the culture supernatant when Serratia marcescens 2170 is grown in the presence of chitin. The gene (cbp) for CBP21 was found to be located in a region 1.5 kb downstream of the chiB gene encoding chitinase B. The cbp gene encodes a polypeptide of 197 amino acids with a calculated size of 21.6 kDa containing a putative signal sequence of 27 amino acids. Comparison of the amino acid sequence of the deduced polypeptide with that of other proteins showed that CBP21 is similar (45.3% amino acid identity) to CHB1 of Streptomyces olivaceoviridis. Purified CBP21 prepared from the periplasmic fraction of Escherichia coli carrying the cloned cbp gene showed its highest binding activity to squid chitin (β-chitin) followed by colloidal chitin and regenerated chitin. Binding of CBP21 to regenerated chitin was affected by pH, in particular, low pH reduced binding activity markedly. The presence of similar chitin binding proteins in the distantly related microorganisms, Streptomyces and Serratia, suggests a wide distribution of this type of chitin binding protein in chitinolytic microorganisms. | |||||
言語 | en | |||||
書誌情報 |
en : Bioscience, Biotechnology, and Biochemistry 巻 62, 号 1, p. 128-135, 発行日 1998 |
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出版者 | ||||||
言語 | en | |||||
出版者 | Japan Society for Bioscience, Biotechnology, and Agrochemistry | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0916-8451 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA10824164 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1271/bbb.62.128 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |