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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

Chitin binding protein (CBP21) in the culture supernatant of Serratia marcescens 2170

http://hdl.handle.net/10191/6331
http://hdl.handle.net/10191/6331
9ec4804c-58f7-4b1a-b038-5e5557e1a0c0
名前 / ファイル ライセンス アクション
07_0007.pdf 07_0007.pdf (1.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-04-23
タイトル
言語 en
タイトル Chitin binding protein (CBP21) in the culture supernatant of Serratia marcescens 2170
言語
言語 eng
キーワード
主題Scheme Other
主題 chitin binding protein
キーワード
主題Scheme Other
主題 Serratia marcescens
キーワード
主題Scheme Other
主題 chitinase
キーワード
主題Scheme Other
主題 nucleotide sequence
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Suzuki, Kazushi

× Suzuki, Kazushi

WEKO 4396

en Suzuki, Kazushi

Search repository
Suzuki, Megumi

× Suzuki, Megumi

WEKO 4397

en Suzuki, Megumi

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Taiyoji, Mayumi

× Taiyoji, Mayumi

WEKO 4398

en Taiyoji, Mayumi

Search repository
Nikaidou, Naoki

× Nikaidou, Naoki

WEKO 4399

en Nikaidou, Naoki

Search repository
Watanabe, Takeshi

× Watanabe, Takeshi

WEKO 4400

en Watanabe, Takeshi

Search repository
著者別名
識別子 4401
識別子Scheme WEKO
姓名 鈴木, 一史
言語 ja
抄録
内容記述タイプ Abstract
内容記述 A chitin binding protein (CBP21) 21 kDa in size, is a major protein in the culture supernatant when Serratia marcescens 2170 is grown in the presence of chitin. The gene (cbp) for CBP21 was found to be located in a region 1.5 kb downstream of the chiB gene encoding chitinase B. The cbp gene encodes a polypeptide of 197 amino acids with a calculated size of 21.6 kDa containing a putative signal sequence of 27 amino acids. Comparison of the amino acid sequence of the deduced polypeptide with that of other proteins showed that CBP21 is similar (45.3% amino acid identity) to CHB1 of Streptomyces olivaceoviridis. Purified CBP21 prepared from the periplasmic fraction of Escherichia coli carrying the cloned cbp gene showed its highest binding activity to squid chitin (β-chitin) followed by colloidal chitin and regenerated chitin. Binding of CBP21 to regenerated chitin was affected by pH, in particular, low pH reduced binding activity markedly. The presence of similar chitin binding proteins in the distantly related microorganisms, Streptomyces and Serratia, suggests a wide distribution of this type of chitin binding protein in chitinolytic microorganisms.
言語 en
書誌情報 en : Bioscience, Biotechnology, and Biochemistry

巻 62, 号 1, p. 128-135, 発行日 1998
出版者
言語 en
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10824164
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1271/bbb.62.128
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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