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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

LysR-type transcriptional regulator ChiR is essential for production of all chitinases and a chitin-binding protein, CBP21, in Serratia marcescens 2170

http://hdl.handle.net/10191/6330
http://hdl.handle.net/10191/6330
38e0f760-6d40-4402-b2dc-91ee092cf81b
名前 / ファイル ライセンス アクション
07_0006.pdf 07_0006.pdf (1.9 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-04-23
タイトル
言語 en
タイトル LysR-type transcriptional regulator ChiR is essential for production of all chitinases and a chitin-binding protein, CBP21, in Serratia marcescens 2170
言語
言語 eng
キーワード
主題Scheme Other
主題 Serratia marcescens
キーワード
主題Scheme Other
主題 LysR-type transcriptional regulator
キーワード
主題Scheme Other
主題 chitinase
キーワード
主題Scheme Other
主題 chitin binding protein
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Suzuki, Kazushi

× Suzuki, Kazushi

WEKO 4334

en Suzuki, Kazushi

Search repository
Uchiyama, Taku

× Uchiyama, Taku

WEKO 4335

en Uchiyama, Taku

Search repository
Suzuki, Megumi

× Suzuki, Megumi

WEKO 4336

en Suzuki, Megumi

Search repository
Nikaidou, Naoki

× Nikaidou, Naoki

WEKO 4337

en Nikaidou, Naoki

Search repository
Regue, Miguel

× Regue, Miguel

WEKO 4338

en Regue, Miguel

Search repository
Watanabe, Takeshi

× Watanabe, Takeshi

WEKO 4339

en Watanabe, Takeshi

Search repository
著者別名
識別子 4340
識別子Scheme WEKO
姓名 鈴木, 一史
言語 ja
抄録
内容記述タイプ Abstract
内容記述 To identify the genes required for chitinase production by Serratia marcescens 2170, various Tn5 mutants somehow defective in chitinase production were isolated in a previous study. In order to identify the mutated gene in one of the chitinase-deficient mutants, N1, DNA regions flanking the Tn5 insertion were cloned and sequenced. Sequence comparison showed that the mutation occurred in the ORF located between chiB and cbp, which encode chitinase B and chitin-binding protein CBP21, respectively. The ORF encodes a 313-amino acid polypeptide which has significant similarity with various LysR-type transcriptional regulators, and thus the gene was designated chiR. Targeted mutagenesis confirmed that disruption of the chiR gene results in the phenotype of N1. Gel mobility shift assays using partially purified ChiR protein demonstrated that this protein specifically binds to the intergenic region between chiR and cbp. These results strongly suggest that ChiR is a LysR-type transcriptional regulator which is essential for production of all chitinases and CBP21.
書誌情報 en : Bioscience, Biotechnology, and Biochemistry

巻 65, 号 2, p. 338-347, 発行日 2001
出版者
言語 en
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10824164
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1271/bbb.65.338
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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