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  1. 0 資料タイプ別
  2. 01 学術雑誌論文
  1. 070 農学部
  2. 10 学術雑誌論文
  3. 10 査読済論文

Proteomic Identification of α-Amylase Isoforms Encoded by RAmy3B/3C from Germinating Rice Seeds

http://hdl.handle.net/10191/6326
http://hdl.handle.net/10191/6326
d69a624e-888e-449e-8c2f-210b99ee4a4e
名前 / ファイル ライセンス アクション
07_0002.pdf 07_0002.pdf (161.9 kB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2008-04-23
タイトル
言語 en
タイトル Proteomic Identification of α-Amylase Isoforms Encoded by RAmy3B/3C from Germinating Rice Seeds
言語
言語 eng
キーワード
主題Scheme Other
主題 α-amylase
キーワード
主題Scheme Other
主題 germinating rice seed
キーワード
主題Scheme Other
主題 RAmy3B
キーワード
主題Scheme Other
主題 RAmy3C
キーワード
主題Scheme Other
主題 proteomics
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Nanjo, Yohei

× Nanjo, Yohei

WEKO 4304

en Nanjo, Yohei

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Asatsuma, Satoru

× Asatsuma, Satoru

WEKO 4305

en Asatsuma, Satoru

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Itoh, Kimiko

× Itoh, Kimiko

WEKO 4306

en Itoh, Kimiko

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Hori, Hidetaka

× Hori, Hidetaka

WEKO 4307

en Hori, Hidetaka

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Mitsui, Toshiaki

× Mitsui, Toshiaki

WEKO 4308

en Mitsui, Toshiaki

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著者別名
識別子 4309
識別子Scheme WEKO
姓名 三ツ井, 敏明
言語 ja
抄録
内容記述タイプ Abstract
内容記述 We isolated and identified 10 α-amylase isoforms by using β-cyclodextrin Sepharose affinity column chromatography and two-dimensional polyacrylamide gel electrophoresis from germinating rice (Oryza sativa L.) seeds. Immunoblots with anti-α-amylase I-1 and II-4 antibodies indicated that 8 isoforms in 10 are distinguishable from α-amylase I-1 and II-4. Peptide mass fingerprinting analysis showed that there exist novel isoforms encoded by RAmy3B and RAmy3C genes. The optimum temperature for enzyme reaction of the RAmy3B and RAmy3C coding isoforms resembled that of α-amylase isoform II-4 (RAmy3D). Furthermore, complex protein polymorphism resulted from a single α-amylase gene was found to occur not only in RAmy3D, but also in RAmy3B.
言語 en
書誌情報 en : Bioscience, Biotechnology, and Biochemistry

巻 68, 号 1, p. 112-118, 発行日 2004
出版者
言語 en
出版者 Japan Society for Bioscience, Biotechnology, and Agrochemistry
ISSN
収録物識別子タイプ ISSN
収録物識別子 0916-8451
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA10824164
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.1271/bbb.68.112
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
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