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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 02 新潟医学会雑誌
  4. 第114巻第10号

Differential display法を用いた新規ケラチンmK 6 irsの同定

http://hdl.handle.net/10191/49189
http://hdl.handle.net/10191/49189
b6d2698e-8969-4612-a60b-4f95f3ca108a
名前 / ファイル ライセンス アクション
114(10)_394-404.pdf 114(10)_394-404.pdf (3.3 MB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2017-11-21
タイトル
タイトル Differential display法を用いた新規ケラチンmK 6 irsの同定
タイトル
言語 en
タイトル Differential display法を用いた新規ケラチンmK 6 irsの同定
言語
言語 jpn
キーワード
主題Scheme Other
主題 differential display
キーワード
主題Scheme Other
主題 keratin intermediate filament
キーワード
主題Scheme Other
主題 inner root sheath
キーワード
主題Scheme Other
主題 ディフェレンシャルディスプレー法
キーワード
主題Scheme Other
主題 ケラチン中間径繊維
キーワード
主題Scheme Other
主題 内毛根鞘
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
その他のタイトル
その他のタイトル Identification of a Novel Keratin mK 6 irs Using a Differential Display
著者 澤田, 壮平

× 澤田, 壮平

WEKO 97580

澤田, 壮平

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著者別名
識別子 97581
識別子Scheme WEKO
姓名 Sawada, Souhei
抄録
内容記述タイプ Abstract
内容記述 During the hair growth cycle, several families of keratin proteins are synthesized sequentially. To investigate the gene expression of different stages of hair cycle is the clue to understand the mechanism of cell differentiation or proliferation. Differential display method is one of the useful strategy to study the gene expression because of its ability to compare multiple RNA populations simultaneously. As a result, I isolated a hitherto unknown gene. The novel gene encodes 2193 nucleotide sequence includes the entire coding region for a protein of 524 amino acids. Northern analysis showed that the transcript detected during the anagen phase is expressed only in skin, but not in other tissues. By screening the Genbank , the deduced amino acid sequence exhibits the highly homology with type II cytokeratins and the secondary structure constitutes the keratin intermediate filaments. In addition, the short regions at the extremities of the rod domain, called the helix initiation motif and the helix termination motif, which specifically conserved among the cytokeratin families are also presents. Moreover, the amino acid composition revealed glycine-rich but low cysteine content in head and tail domains, exclude the possibility of its character as a hair keratin family. By immuno-histochemistry, the mk6irs expression occurs at inner root sheath in the late-anagen phase, predominantly in the Huxley's and Henle's layer of mouse hair follicles.
書誌情報 新潟医学会雑誌
en : 新潟医学会雑誌

巻 114, 号 10, p. 394-404, 発行日 2000-10
出版者
出版者 新潟医学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 00290440
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AN00182415
著者版フラグ
値 publisher
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