{"_buckets": {"deposit": "6f449ac1-14b2-4aa8-ab90-405129eea8cf"}, "_deposit": {"id": "15192", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "15192"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00015192"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Identification of a Novel Keratin mK 6 irs Using a Differential Display"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "404", "bibliographicPageStart": "394", "bibliographicVolumeNumber": "114", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "During the hair growth cycle, several families of keratin proteins are synthesized sequentially. To investigate the gene expression of different stages of hair cycle is the clue to understand the mechanism of cell differentiation or proliferation. Differential display method is one of the useful strategy to study the gene expression because of its ability to compare multiple RNA populations simultaneously. As a result, I isolated a hitherto unknown gene. The novel gene encodes 2193 nucleotide sequence includes the entire coding region for a protein of 524 amino acids. Northern analysis showed that the transcript detected during the anagen phase is expressed only in skin, but not in other tissues. By screening the Genbank , the deduced amino acid sequence exhibits the highly homology with type II cytokeratins and the secondary structure constitutes the keratin intermediate filaments. In addition, the short regions at the extremities of the rod domain, called the helix initiation motif and the helix termination motif, which specifically conserved among the cytokeratin families are also presents. Moreover, the amino acid composition revealed glycine-rich but low cysteine content in head and tail domains, exclude the possibility of its character as a hair keratin family. By immuno-histochemistry, the mk6irs expression occurs at inner root sheath in the late-anagen phase, predominantly in the Huxley\u0027s and Henle\u0027s layer of mouse hair follicles.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "97581", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Sawada, Souhei"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u533b\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u6fa4\u7530, \u58ee\u5e73"}], "nameIdentifiers": [{"nameIdentifier": "97580", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-07"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "114(10)_394-404.pdf", "filesize": [{"value": "3.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3300000.0, "url": {"label": "114(10)_394-404.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/15192/files/114(10)_394-404.pdf"}, "version_id": "31acb9f5-67a0-4a21-9ef1-14da253b839e"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "differential display", "subitem_subject_scheme": "Other"}, {"subitem_subject": "keratin intermediate filament", "subitem_subject_scheme": "Other"}, {"subitem_subject": "inner root sheath", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30c7\u30a3\u30d5\u30a7\u30ec\u30f3\u30b7\u30e3\u30eb\u30c7\u30a3\u30b9\u30d7\u30ec\u30fc\u6cd5", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u30b1\u30e9\u30c1\u30f3\u4e2d\u9593\u5f84\u7e4a\u7dad", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u5185\u6bdb\u6839\u9798", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Differential display\u6cd5\u3092\u7528\u3044\u305f\u65b0\u898f\u30b1\u30e9\u30c1\u30f3mK 6 irs\u306e\u540c\u5b9a", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Differential display\u6cd5\u3092\u7528\u3044\u305f\u65b0\u898f\u30b1\u30e9\u30c1\u30f3mK 6 irs\u306e\u540c\u5b9a"}, {"subitem_title": "Differential display\u6cd5\u3092\u7528\u3044\u305f\u65b0\u898f\u30b1\u30e9\u30c1\u30f3mK 6 irs\u306e\u540c\u5b9a", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/538/1082"], "permalink_uri": "http://hdl.handle.net/10191/49189", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-11-21"}, "publish_date": "2017-11-21", "publish_status": "0", "recid": "15192", "relation": {}, "relation_version_is_last": true, "title": ["Differential display\u6cd5\u3092\u7528\u3044\u305f\u65b0\u898f\u30b1\u30e9\u30c1\u30f3mK 6 irs\u306e\u540c\u5b9a"], "weko_shared_id": null}