{"_buckets": {"deposit": "6f449ac1-14b2-4aa8-ab90-405129eea8cf"}, "_deposit": {"id": "15192", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "15192"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00015192", "sets": ["456", "1082"]}, "item_7_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "Identification of a Novel Keratin mK 6 irs Using a Differential Display"}]}, "item_7_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2000-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "404", "bibliographicPageStart": "394", "bibliographicVolumeNumber": "114", "bibliographic_titles": [{"bibliographic_title": "新潟医学会雑誌"}, {"bibliographic_title": "新潟医学会雑誌", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "During the hair growth cycle, several families of keratin proteins are synthesized sequentially. To investigate the gene expression of different stages of hair cycle is the clue to understand the mechanism of cell differentiation or proliferation. Differential display method is one of the useful strategy to study the gene expression because of its ability to compare multiple RNA populations simultaneously. As a result, I isolated a hitherto unknown gene. The novel gene encodes 2193 nucleotide sequence includes the entire coding region for a protein of 524 amino acids. Northern analysis showed that the transcript detected during the anagen phase is expressed only in skin, but not in other tissues. By screening the Genbank , the deduced amino acid sequence exhibits the highly homology with type II cytokeratins and the secondary structure constitutes the keratin intermediate filaments. In addition, the short regions at the extremities of the rod domain, called the helix initiation motif and the helix termination motif, which specifically conserved among the cytokeratin families are also presents. Moreover, the amino acid composition revealed glycine-rich but low cysteine content in head and tail domains, exclude the possibility of its character as a hair keratin family. By immuno-histochemistry, the mk6irs expression occurs at inner root sheath in the late-anagen phase, predominantly in the Huxley\u0027s and Henle\u0027s layer of mouse hair follicles.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "97581", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Sawada, Souhei"}]}]}, "item_7_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟医学会"}]}, "item_7_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "書誌レコードID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "澤田, 壮平"}], "nameIdentifiers": [{"nameIdentifier": "97580", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-07"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "114(10)_394-404.pdf", "filesize": [{"value": "3.3 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 3300000.0, "url": {"label": "114(10)_394-404.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/15192/files/114(10)_394-404.pdf"}, "version_id": "31acb9f5-67a0-4a21-9ef1-14da253b839e"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "differential display", "subitem_subject_scheme": "Other"}, {"subitem_subject": "keratin intermediate filament", "subitem_subject_scheme": "Other"}, {"subitem_subject": "inner root sheath", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ディフェレンシャルディスプレー法", "subitem_subject_scheme": "Other"}, {"subitem_subject": "ケラチン中間径繊維", "subitem_subject_scheme": "Other"}, {"subitem_subject": "内毛根鞘", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Differential display法を用いた新規ケラチンmK 6 irsの同定", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Differential display法を用いた新規ケラチンmK 6 irsの同定"}, {"subitem_title": "Differential display法を用いた新規ケラチンmK 6 irsの同定", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["456", "1082"], "permalink_uri": "http://hdl.handle.net/10191/49189", "pubdate": {"attribute_name": "公開日", "attribute_value": "2017-11-21"}, "publish_date": "2017-11-21", "publish_status": "0", "recid": "15192", "relation": {}, "relation_version_is_last": true, "title": ["Differential display法を用いた新規ケラチンmK 6 irsの同定"], "weko_shared_id": null}