WEKO3
アイテム
Differential display法を用いた新規ケラチンmK 6 irsの同定
http://hdl.handle.net/10191/49189
http://hdl.handle.net/10191/49189b6d2698e-8969-4612-a60b-4f95f3ca108a
名前 / ファイル | ライセンス | アクション |
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114(10)_394-404.pdf (3.3 MB)
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Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
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公開日 | 2017-11-21 | |||||
タイトル | ||||||
タイトル | Differential display法を用いた新規ケラチンmK 6 irsの同定 | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Differential display法を用いた新規ケラチンmK 6 irsの同定 | |||||
言語 | ||||||
言語 | jpn | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | differential display | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | keratin intermediate filament | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | inner root sheath | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ディフェレンシャルディスプレー法 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | ケラチン中間径繊維 | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | 内毛根鞘 | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_6501 | |||||
タイプ | departmental bulletin paper | |||||
その他のタイトル | ||||||
その他のタイトル | Identification of a Novel Keratin mK 6 irs Using a Differential Display | |||||
著者 |
澤田, 壮平
× 澤田, 壮平 |
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著者別名 | ||||||
識別子 | 97581 | |||||
識別子Scheme | WEKO | |||||
姓名 | Sawada, Souhei | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | During the hair growth cycle, several families of keratin proteins are synthesized sequentially. To investigate the gene expression of different stages of hair cycle is the clue to understand the mechanism of cell differentiation or proliferation. Differential display method is one of the useful strategy to study the gene expression because of its ability to compare multiple RNA populations simultaneously. As a result, I isolated a hitherto unknown gene. The novel gene encodes 2193 nucleotide sequence includes the entire coding region for a protein of 524 amino acids. Northern analysis showed that the transcript detected during the anagen phase is expressed only in skin, but not in other tissues. By screening the Genbank , the deduced amino acid sequence exhibits the highly homology with type II cytokeratins and the secondary structure constitutes the keratin intermediate filaments. In addition, the short regions at the extremities of the rod domain, called the helix initiation motif and the helix termination motif, which specifically conserved among the cytokeratin families are also presents. Moreover, the amino acid composition revealed glycine-rich but low cysteine content in head and tail domains, exclude the possibility of its character as a hair keratin family. By immuno-histochemistry, the mk6irs expression occurs at inner root sheath in the late-anagen phase, predominantly in the Huxley's and Henle's layer of mouse hair follicles. | |||||
書誌情報 |
新潟医学会雑誌 en : 新潟医学会雑誌 巻 114, 号 10, p. 394-404, 発行日 2000-10 |
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出版者 | ||||||
出版者 | 新潟医学会 | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 00290440 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AN00182415 | |||||
著者版フラグ | ||||||
値 | publisher |