ログイン
言語:

WEKO3

  • トップ
  • ランキング
To
lat lon distance
To

Field does not validate



インデックスリンク

インデックスツリー

メールアドレスを入力してください。

WEKO

One fine body…

WEKO

One fine body…

アイテム

{"_buckets": {"deposit": "ec12a2bc-b3d1-444f-a393-f0bed7020500"}, "_deposit": {"id": "1506", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "1506"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00001506", "sets": ["454", "425"]}, "author_link": ["4071", "4072", "4073", "4074", "4075", "4076", "4077", "4078", "4079"], "control_number": "1506", "item_1627363077551": {"attribute_name": "出版タイプ", "attribute_value_mlt": [{"subitem_version_resource": "http://purl.org/coar/version/c_970fb48d4fbd8a85", "subitem_version_type": "VoR"}]}, "item_5_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2007-11", "bibliographicIssueDateType": "Issued"}, "bibliographicPageEnd": "59", "bibliographicPageStart": "51", "bibliographicVolumeNumber": "2", "bibliographic_titles": [{"bibliographic_title": "Analytical Chemistry Insights", "bibliographic_titleLang": "en"}]}]}, "item_5_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the detection and characterization of proteases and protease inhibitors. Isoelectric focusing (IEF) agarose gels with pH gradients were employed for separation in the fi rst-dimension and sodium dodecyl sulfate (SDS)-polyacrylamide gel copolymerized with gelatin used for the second dimension. Proteases and protease inhibitors separated by IEF gel were applied on the second gel without trichloroacetic acid (TCA) fi xation. Protease activity in the 2D-gel was visualized as transparent spots where gelatin substrate was digested after commassie brilliant blue (CBB) staining. Some of the transparent spots from the skin mucus extract of rainbow trout were determined to be a cysteine protease through use of E-64 or CA-074. In the reverse zymography technique, the gel was incubated with papain solution at 37 ºC for 18 h. Cysteine protease inhibitors from broad bean seeds were detected as clear blue spots after CBB staining. The amino (N-) terminal sequences of four papain inhibitor spots thus detected were demonstrated to be identical to that of favin β chain, a broad bean lectin. Taken together, our system can be considered to be an effi cient technique for discovering and characterizing new proteases and protease inhibitors in biological samples. This is the fi rst report describing a 2D-gel system of zymography and reverse zymography.", "subitem_description_type": "Abstract"}]}, "item_5_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "Libertas Academica"}]}, "item_5_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "http://doi.org/10.4137/117739010700200011", "subitem_relation_type_select": "DOI"}}]}, "item_5_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "Copyright(C) 2007 Libertas Academica"}]}, "item_5_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_5_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "1177-3901", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Saitoh, Eiichi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4071", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Yamamoto, Shinya", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4072", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Okamoto, Eishiro", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4073", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hayakawa, Yoshimi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4074", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Hoshino, Takashi", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4075", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Sato, Ritsuko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4076", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Isemura, Satoko", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4077", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Ohtsubo, Sadami", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4078", "nameIdentifierScheme": "WEKO"}]}, {"creatorNames": [{"creatorName": "Taniguchi, Masayuki", "creatorNameLang": "en"}], "nameIdentifiers": [{"nameIdentifier": "4079", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-07-29"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "2_51-59.pdf", "filesize": [{"value": "1.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1400000.0, "url": {"label": "2_51-59.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/1506/files/2_51-59.pdf"}, "version_id": "781596d8-9ec7-4d0f-9c06-e406d7d4215a"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "Broad bean lectin", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cysteine proteases", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Cysteine protease inhibitors", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Reverse zymography", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Two-dimensional gel electrophoresis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "Zymography", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "journal article", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography", "subitem_title_language": "en"}]}, "item_type_id": "5", "owner": "1", "path": ["454", "425"], "permalink_uri": "http://hdl.handle.net/10191/30169", "pubdate": {"attribute_name": "PubDate", "attribute_value": "2014-11-12"}, "publish_date": "2014-11-12", "publish_status": "0", "recid": "1506", "relation": {}, "relation_version_is_last": true, "title": ["Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography"], "weko_shared_id": -1}
  1. 060 工学部
  2. 10 学術雑誌論文
  3. 10 査読済論文
  1. 0 資料タイプ別
  2. 01 学術雑誌論文

Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography

http://hdl.handle.net/10191/30169
http://hdl.handle.net/10191/30169
b4924d44-b3b2-4a85-a712-03df2fa29a3e
名前 / ファイル ライセンス アクション
2_51-59.pdf 2_51-59.pdf (1.4 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-11-12
タイトル
言語 en
タイトル Identification of Cysteine Proteases and Screening of Cysteine Protease Inhibitors in Biological Samples by a Two-Dimensional Gel System of Zymography and Reverse Zymography
言語
言語 eng
キーワード
主題Scheme Other
主題 Broad bean lectin
キーワード
主題Scheme Other
主題 Cysteine proteases
キーワード
主題Scheme Other
主題 Cysteine protease inhibitors
キーワード
主題Scheme Other
主題 Reverse zymography
キーワード
主題Scheme Other
主題 Two-dimensional gel electrophoresis
キーワード
主題Scheme Other
主題 Zymography
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
著者 Saitoh, Eiichi

× Saitoh, Eiichi

WEKO 4071

en Saitoh, Eiichi

Search repository
Yamamoto, Shinya

× Yamamoto, Shinya

WEKO 4072

en Yamamoto, Shinya

Search repository
Okamoto, Eishiro

× Okamoto, Eishiro

WEKO 4073

en Okamoto, Eishiro

Search repository
Hayakawa, Yoshimi

× Hayakawa, Yoshimi

WEKO 4074

en Hayakawa, Yoshimi

Search repository
Hoshino, Takashi

× Hoshino, Takashi

WEKO 4075

en Hoshino, Takashi

Search repository
Sato, Ritsuko

× Sato, Ritsuko

WEKO 4076

en Sato, Ritsuko

Search repository
Isemura, Satoko

× Isemura, Satoko

WEKO 4077

en Isemura, Satoko

Search repository
Ohtsubo, Sadami

× Ohtsubo, Sadami

WEKO 4078

en Ohtsubo, Sadami

Search repository
Taniguchi, Masayuki

× Taniguchi, Masayuki

WEKO 4079

en Taniguchi, Masayuki

Search repository
抄録
内容記述タイプ Abstract
内容記述 We have developed a two-dimensional (2D-) gel system of zymography and reverse zymography for the detection and characterization of proteases and protease inhibitors. Isoelectric focusing (IEF) agarose gels with pH gradients were employed for separation in the fi rst-dimension and sodium dodecyl sulfate (SDS)-polyacrylamide gel copolymerized with gelatin used for the second dimension. Proteases and protease inhibitors separated by IEF gel were applied on the second gel without trichloroacetic acid (TCA) fi xation. Protease activity in the 2D-gel was visualized as transparent spots where gelatin substrate was digested after commassie brilliant blue (CBB) staining. Some of the transparent spots from the skin mucus extract of rainbow trout were determined to be a cysteine protease through use of E-64 or CA-074. In the reverse zymography technique, the gel was incubated with papain solution at 37 ºC for 18 h. Cysteine protease inhibitors from broad bean seeds were detected as clear blue spots after CBB staining. The amino (N-) terminal sequences of four papain inhibitor spots thus detected were demonstrated to be identical to that of favin β chain, a broad bean lectin. Taken together, our system can be considered to be an effi cient technique for discovering and characterizing new proteases and protease inhibitors in biological samples. This is the fi rst report describing a 2D-gel system of zymography and reverse zymography.
書誌情報 en : Analytical Chemistry Insights

巻 2, p. 51-59, 発行日 2007-11
出版者
出版者 Libertas Academica
ISSN
収録物識別子タイプ ISSN
収録物識別子 1177-3901
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.4137/117739010700200011
権利
権利情報 Copyright(C) 2007 Libertas Academica
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
戻る
0
views
See details
Views

Versions

Ver.1 2021-03-01 20:59:34.116908
Show All versions

Share

Mendeley Twitter Facebook Print Addthis

Cite as

エクスポート

OAI-PMH
  • OAI-PMH JPCOAR
  • OAI-PMH DublinCore
  • OAI-PMH DDI
Other Formats
  • JSON
  • BIBTEX

Confirm


Powered by WEKO3


Powered by WEKO3