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Expression in Escherichia coli of the Bacillus circulans WL-12 Ｓtructural Gene for β-1,3-Glucanase A
http://hdl.handle.net/10191/6329
http://hdl.handle.net/10191/63291e382991-22cf-4b2d-8432-57781d25ba94
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
07_0005.pdf (763.1 kB)
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| Item type | 学術雑誌論文 / Journal Article(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2008-04-23 | |||||
| タイトル | ||||||
| タイトル | Expression in Escherichia coli of the Bacillus circulans WL-12 Ｓtructural Gene for β-1,3-Glucanase A | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | journal article | |||||
| 著者 |
Watanabe, Takeshi
× Watanabe, Takeshi× Yahata, Naokazu× Nakamura, Yasushi× Muramoto, Yoshimitsu× Suzuki, Kazushi× Kamimiya, Shusei× Tanaka, Hirosato |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 4033 | |||||
| 姓名 | 鈴木, 一史 | |||||
| 言語 | ja | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Bacillus circulans WL-12, a yeast and fungal cell wall lytic bacterium, secretes a variety of polysaccharide degrading enzymes into the culture medium. When β-1,3-glucanase was induced with pachyman, a β-1,3-glucose polymer obtained from the tree fungus Poria cocus Wolf, six distinct active molecules of the enzyme with different molecular weights were detected in the culture supernatant of this bacterium. Molecular cloning of one of the β-1,3-glucanase genes into E. coli was achieved by transforming E. coli HB101 cells with recombinant plasmids composed of chromosomal DNA fragments prepared from B. circulans WL-12 and the plasmid vector pUC 19. A recombinant plasmid containing 4.4kb of inserted DNA in the PstI site of pUC 19, designated as pNT003, conferred the ability to degrade pachyman on E. coli cells. The presence of pNT003 was harmful for E. coli cells and caused cell lysis, especially at higher temperatures of cultivation. β-1,3-Glucanase activity detected in E. coli was mainly recovered in the periplasmic fraction when cell lysis did not occur. SDS-PAGE analysis revealed that the periplasmic fraction contained four active molecules of β-1,3-glucanase which corresponded to four of the six active molecules produced by B. circulans WL-12. | |||||
| 言語 | en | |||||
| 書誌情報 |
en : Agricultural and Biolobical Chemistry 巻 53, 号 7, p. 1759-1767, 発行日 1989 |
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| 出版者 | ||||||
| 出版者 | Agricultural Chemical Society of Japan | |||||
| 言語 | en | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 0002-1369 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00515312 | |||||
| DOI | ||||||
| 識別子タイプ | DOI | |||||
| 関連識別子 | http://doi.org/10.1271/bbb1961.53.1759 | |||||
| 出版タイプ | ||||||
| 出版タイプ | VoR | |||||
| 出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
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Cite as
Watanabe, Takeshi, Yahata, Naokazu, Nakamura, Yasushi, Muramoto, Yoshimitsu, Suzuki, Kazushi, Kamimiya, Shusei, Tanaka, Hirosato, 1989, Expression in Escherichia coli of the Bacillus circulans WL-12 Ｓtructural Gene for β-1,3-Glucanase A: Agricultural Chemical Society of Japan, 1759–1767 p.
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