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Ultrastructural Alteration of Osteoclasts Treated with Brefeldin A and Wortmannin
http://hdl.handle.net/10191/5700
http://hdl.handle.net/10191/5700e3e4f486-8687-4e17-9ba1-cc9db4765eef
名前 / ファイル | ライセンス | アクション |
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Item type | 学術雑誌論文 / Journal Article(1) | |||||
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公開日 | 2008-04-08 | |||||
タイトル | ||||||
タイトル | Ultrastructural Alteration of Osteoclasts Treated with Brefeldin A and Wortmannin | |||||
言語 | en | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Osteoclast | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Brefeldin A | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Wortmannin | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Golgi apparatus | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | Ruffled border | |||||
資源タイプ | ||||||
資源タイプ識別子 | http://purl.org/coar/resource_type/c_6501 | |||||
資源タイプ | journal article | |||||
著者 |
Izumi, Naoya
× Izumi, Naoya× Amizuka, Norio× Oda, Kimimitsu× Misumi, Yoshio× Ikehara, Yukio× Ozawa, Hidehiro |
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著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 4007 | |||||
姓名 | 泉, 直也 | |||||
言語 | ja | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 4008 | |||||
姓名 | 網塚, 憲生 | |||||
言語 | ja | |||||
著者別名 | ||||||
識別子Scheme | WEKO | |||||
識別子 | 4009 | |||||
姓名 | 織田, 公光 | |||||
言語 | ja | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | Osteoclasts secrete protons and lysosomal enzymes through ruffled borders, resulting in demineralization and degradation of bone matrix. Golgi apparatus is thought to play a key role in this process. In order to elucidate the specific functions of Golgi apparatus in osteoclasts, we observed the ultrastructural and cytochemical changes resulting from the administration of brefeldin A (BFA), an inhibitor of Golgi structures, and wortmannin (WT), an inhibitor of phosphatidylinositol 3-kinase (PI3-kinase). When treated with BFA, osteoclasts showed a dissociated Golgi apparatus, while the peanut agglutinin (PNA)-positive ruffled borders were poorly developed or disappeared. Moreover, the acid phosphatase (ACPase) activity was somewhat reduced. WT-treated osteoclasts, on the other hand, exhibited no ultrastructural alteration of the Golgi apparatus, but were marked by the disappearance of PNApositive ruffled borders, and a consequent increase in the number of ACPase-positive vesicles and vacuoles in the cytoplasm. These results indicate that the Golgi apparatus of osteoclasts not only plays an important role in the formation of lysosomal enzymes but also the supply of ruffled border membranes. Furthermore, PI 3-kinase appears to be involved in the transport of lysosomal enzymes and the maintenance of ruffled borders. | |||||
言語 | en | |||||
書誌情報 |
en : Acta histochemica et cytochemica 巻 32, 号 5, p. 393-405, 発行日 1999 |
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出版者 | ||||||
出版者 | 日本組織細胞化学会 | |||||
言語 | ja | |||||
ISSN | ||||||
収録物識別子タイプ | ISSN | |||||
収録物識別子 | 0044-5991 | |||||
書誌レコードID | ||||||
収録物識別子タイプ | NCID | |||||
収録物識別子 | AA00508022 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | http://doi.org/10.1267/ahc.32.393 | |||||
出版タイプ | ||||||
出版タイプ | VoR | |||||
出版タイプResource | http://purl.org/coar/version/c_970fb48d4fbd8a85 | |||||
著者版フラグ | ||||||
値 | publisher |