{"_buckets": {"deposit": "77a6ce3a-e929-49b5-b8bf-817e75019d36"}, "_deposit": {"id": "14658", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "14658"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00014658"}, "item_7_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "Functional Difference Between \u03b52 and \u03b54 Subunits of NMDA Receptor Channel in the Developmental Mouse Brain"}]}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2001-10", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "10", "bibliographicPageEnd": "527", "bibliographicPageStart": "518", "bibliographicVolumeNumber": "115", "bibliographic_titles": [{"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c"}, {"bibliographic_title": "\u65b0\u6f5f\u533b\u5b66\u4f1a\u96d1\u8a8c", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The N -methyl-D-aspartate (NMDA) subtype of the glutamate receptor (GluR) channel plays roles in synaptic plasticity as a molecular coincident detector and in neuronal pattern formation during development. NMDA receptor channels are composed of the GluR\u03b5 (NR 2) and GluR\u03b6 (NR 1) subunits. There are four GluR\u03b5 subunit genes, although GluR\u03b6 subunit variants are derived from a single gene. The molecular composition of NMDA receptor channels varies depending on the location and developmental stage of brain. At the embryonic stage, GluR\u03b5 2 mRNA is expressed in the entire brain, while GluR\u03b5 4 mRNA is expressed only in the diencephalon and brainstem. Therefore, I focused on the GluR\u03b5 2 and GluR\u03b5 4 subunits to determine their functions during development. I produced a mutant mouse line (\u03b5 4-E2), which expressed GluR\u03b5 2 protein under the GluR\u03b5 4 gene promoter, by inserting \u03b5 2 cDNA into GluR\u03b5 4 subunit gene with the homologous recombination. In this mutant mice, I found GluR\u03b5 2 appeared instead of GluR\u03b5 4, and compared its behavioral phenotype with that of the GluR\u03b5 4 knockout mouse. The\u03b5 4-E2 mutant mice showed a significantly lower spontaneous activity than that of the GluR\u03b5 4 knockout mice. On the other hand, there was no significant difference in the spontaneous activity between the GluR\u03b5 4 mutant mice and wild-type mice. These results suggest that the GluR\u03b5 2 and GluR\u03b5 4 are distinct in functional properties and GluR\u03b5 2 cannot compensate the function of GluR\u03b5 4.", "subitem_description_type": "Abstract"}]}, "item_7_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "94614", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "Kakizaki, Toshikazu"}]}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u533b\u5b66\u4f1a"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AN00182415", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "00290440", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "\u67ff\u5d0e, \u5229\u548c"}], "nameIdentifiers": [{"nameIdentifier": "94613", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-07"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "115(10)_518-527.pdf", "filesize": [{"value": "1.8 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1800000.0, "url": {"label": "115(10)_518-527.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/14658/files/115(10)_518-527.pdf"}, "version_id": "c6fa775c-8783-463a-a2c0-b0d84ce3de9c"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "NMDA receptor channel", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GluR\u03b5 2", "subitem_subject_scheme": "Other"}, {"subitem_subject": "GluR\u03b5 4", "subitem_subject_scheme": "Other"}, {"subitem_subject": "homologous recombination", "subitem_subject_scheme": "Other"}, {"subitem_subject": "spontaneous activity", "subitem_subject_scheme": "Other"}, {"subitem_subject": "NMDA\u53d7\u5bb9\u4f53\u30c1\u30e3\u30cd\u30eb", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u76f8\u540c\u7d44\u63db\u3048", "subitem_subject_scheme": "Other"}, {"subitem_subject": "\u81ea\u767a\u7684\u6d3b\u52d5\u6027", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "jpn"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "\u8133\u767a\u9054\u6642\u671f\u306b\u767a\u73fe\u3059\u308bNMDA\u53d7\u5bb9\u4f53\u30c1\u30e3\u30cd\u30eb\u03b52, \u03b54\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u6a5f\u80fd\u7684\u5dee\u9055", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "\u8133\u767a\u9054\u6642\u671f\u306b\u767a\u73fe\u3059\u308bNMDA\u53d7\u5bb9\u4f53\u30c1\u30e3\u30cd\u30eb\u03b52, \u03b54\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u6a5f\u80fd\u7684\u5dee\u9055"}, {"subitem_title": "\u8133\u767a\u9054\u6642\u671f\u306b\u767a\u73fe\u3059\u308bNMDA\u53d7\u5bb9\u4f53\u30c1\u30e3\u30cd\u30eb\u03b52, \u03b54\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u6a5f\u80fd\u7684\u5dee\u9055", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/538/1070"], "permalink_uri": "http://hdl.handle.net/10191/48427", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2017-11-09"}, "publish_date": "2017-11-09", "publish_status": "0", "recid": "14658", "relation": {}, "relation_version_is_last": true, "title": ["\u8133\u767a\u9054\u6642\u671f\u306b\u767a\u73fe\u3059\u308bNMDA\u53d7\u5bb9\u4f53\u30c1\u30e3\u30cd\u30eb\u03b52, \u03b54\u30b5\u30d6\u30e6\u30cb\u30c3\u30c8\u306e\u6a5f\u80fd\u7684\u5dee\u9055"], "weko_shared_id": null}