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The Involvement of Reactive Oxygen Species in Autophagy Regulation by Amino Acids
http://hdl.handle.net/10191/27214
http://hdl.handle.net/10191/272143f4234ff-bded-471f-9f93-1ba7439f6ff3
| 名前 / ファイル | ライセンス | アクション |
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本文 (2.8 MB)
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要旨 (192.6 kB)
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| Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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| 公開日 | 2014-05-16 | |||||
| タイトル | ||||||
| タイトル | The Involvement of Reactive Oxygen Species in Autophagy Regulation by Amino Acids | |||||
| タイトル | ||||||
| タイトル | The Involvement of Reactive Oxygen Species in Autophagy Regulation by Amino Acids | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_46ec | |||||
| タイプ | thesis | |||||
| その他のタイトル | ||||||
| その他のタイトル | アミノ酸によるオートファジー調節における活性酸素種の関与 | |||||
| 著者 |
Angcajas, Aileen Bahalla
× Angcajas, Aileen Bahalla |
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| 著者別名 | ||||||
| 識別子Scheme | WEKO | |||||
| 識別子 | 50275 | |||||
| 姓名 | アンカハス, アイリーン バハラ | |||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | Autophagy, an intracellular bulk degradation process induced by starvation, is regulated by nutrients and different conditions. Reactive oxygen species (ROS) critically modulates autophagy in response to various cellular stresses. In eukaryotic cell, starvation prompts induction of autophagy and during this condition, intracellular ROS level increases. Hence, ROS is considered as a good signaling molecule in autophagy. Amino acid is a known autophagy regulator. However, the significance of ROS production by amino acids to their effect on autophagy has yet to be explored. In the present work, we analyzed the effects of individual amino acids on the intracellular ROS level and autophagy in H4-II-E cells. ROS measurement was performed in Spectrofluorimeter using the fluorescent probe 2', 7'-dichlorofluorescein diacetate (DCFDA). The cytosolic LC3 ratio (LC3-IIs/LC3-I) was employed as a suitable quantitative index of autophagic flux. LC3 signal was detected by Western blotting and quantified by densitometric analysis. CAA mixture showed significant suppression of autophagy. Furthermore, all the amino acid mixtures suppressed the ROS production significantly. Most individual RegAA showed suppressive effects on ROS production and autophagy. This scenario explains the ROS production suppression observed form RegAA mixture. Interestingly, arginine, a NonRegAA showed significant autophagy suppression but its ROS production was greatly stimulated. Inhibitor study using rapamycin and wortmannin showed starvation is partially Class III PI3K dependent. Furthermore, it also suggested that ROS production involvement in the autophagy regulation by CAA and Leu (a RegAA) may also be Class III PI3K dependent. This implies the participation of ROS as signaling molecule in the autophagy regulation by CAA and Leu. Finally, ROS production by arginine exhibited partial mTORC1 pathway dependence; however, its autophagy regulation may involve another pathway. | |||||
| 内容記述 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 学位の種類: 博士（学術）. 報告番号: 甲第3905号. 学位記番号: 新大院博（学）甲第202号. 学位授与年月日: 平成26年3月24日 | |||||
| 書誌情報 | p. 1-88, 発行日 2014-03-24 | |||||
| 出版者 | ||||||
| 出版者 | 新潟大学 | |||||
| 著者版フラグ | ||||||
| 値 | ETD | |||||
| 学位名 | ||||||
| 学位名 | 博士（学術） | |||||
| 学位授与機関 | ||||||
| 学位授与機関名 | 新潟大学 | |||||
| 学位授与年月日 | ||||||
| 学位授与年月日 | 2014-03-24 | |||||
| 学位授与番号 | ||||||
| 学位授与番号 | 13101甲第3905号 | |||||
| 学位記番号 | ||||||
| 内容記述タイプ | Other | |||||
| 内容記述 | 新大院博（学）甲第202号 | |||||
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Cite as
Angcajas, Aileen Bahalla, 2014, The Involvement of Reactive Oxygen Species in Autophagy Regulation by Amino Acids: 新潟大学, p. 1–88.
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