{"created":"2021-03-01T06:13:17.797933+00:00","id":9235,"links":{},"metadata":{"_buckets":{"deposit":"3d3d3aa0-f676-469d-8fcf-dc98da84bb62"},"_deposit":{"id":"9235","owners":[],"pid":{"revision_id":0,"type":"depid","value":"9235"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00009235","sets":["453:456","471:537:538:928"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Isoform-dependent ApoE Metabolism Regulated by Lipoprotein Receptor LR11/SorLA"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2013-08","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"8","bibliographicPageEnd":"415","bibliographicPageStart":"403","bibliographicVolumeNumber":"127","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"},{"bibliographic_title":"新潟医学会雑誌","bibliographic_titleLang":"en"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"アルツハイマー病(Alzheimer disease:AD)は最も頻度の高い認知症であり, その主要な病理変化の一つにβアミロイド(Aβ)の蓄積がある. ADの最大の遺伝的リスクとしてAPOE遺伝子多型が知られている. Aβは, その代謝システムとしてアポリポ蛋白E(ApoE)と複合体を形成し細胞内に取り込まれる機序が知られている. LR11/SorLAはAβの産生・代謝を調節するリポ蛋白受容体であり, その細胞外領域にApoEと結合するドメインを有している. 本研究では, 全長型LR11(full-length LR11：FL-LR11)がisoform依存性にApoEを細胞内に取り込む可能性について培養細胞を用いて検討した. HEK293T細胞にApoE-GFPを遺伝子導入し, その培養液をFL-LR11を導入したN2a細胞に添加し, 分泌ApoE-GFPのN2a細胞への取り込みを検討した. N2a細胞を共焦点レーザー顕微鏡で観察すると, 培養液中のApoE-GFPがLR11を介して細胞内に取り込まれた. ApoE2, E3, E4を遺伝子導入したドナーHEK293T細胞とFL-LR11を遺伝子導入したレシピエントN2a細胞の共培養実験を行ったところ, LR11の強制発現により分泌ApoE3及びApoE4のレシピエントN2a細胞内への取り込みが亢進した.一方で, 分泌ApoE2ではレシピエントN2a細胞への取り込みの亢進は認めなかった. 共免疫沈降解析を行ったところ, ApoEとLR11は細胞の内外で複合体を形成した. ApoE-LR11複合体形成量は, ApoE4 > ApoE3 > ApoE2の順で増加していた. 本研究により, FL-LR11はApoE isoform 依存性にApoEと結合し, その親和性に応じてApoEを細胞内に取り込むことが示唆された.","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"64169","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Yajima, Ryuji"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"矢島, 隆二"}],"nameIdentifiers":[{"nameIdentifier":"64168","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-06"}],"displaytype":"detail","filename":"127(8)_403-415.pdf","filesize":[{"value":"1.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"127(8)_403-415.pdf","url":"https://niigata-u.repo.nii.ac.jp/record/9235/files/127(8)_403-415.pdf"},"version_id":"c805fa02-6413-4e4c-a2a6-de2df805c627"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"アルツハイマー病","subitem_subject_scheme":"Other"},{"subitem_subject":"LR11/SorLA","subitem_subject_scheme":"Other"},{"subitem_subject":"βアミロイド","subitem_subject_scheme":"Other"},{"subitem_subject":"アポリポ蛋白E","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"リポ蛋白受容体LR11/SorLAによるisoform依存性ApoE代謝調節機構の解析","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"リポ蛋白受容体LR11/SorLAによるisoform依存性ApoE代謝調節機構の解析"},{"subitem_title":"リポ蛋白受容体LR11/SorLAによるisoform依存性ApoE代謝調節機構の解析","subitem_title_language":"en"}]},"item_type_id":"7","owner":"1","path":["456","928"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-01-04"},"publish_date":"2016-01-04","publish_status":"0","recid":"9235","relation_version_is_last":true,"title":["リポ蛋白受容体LR11/SorLAによるisoform依存性ApoE代謝調節機構の解析"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T03:43:22.987724+00:00"}