@article{oai:niigata-u.repo.nii.ac.jp:00006663,
 author = {Ikarashi, Toshihiko},
 issue = {2},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Oct},
 note = {The histochemical analyses of glycoproteins were performed on specimens of endocervical type adenocarcinoma of uterine cervical carcinoma (EA) (40 cases) and normal cervices (19 cases) by the following methods: (1) Modified Schiff stains for sialates, including both Periodic Acid-Thionin Schiff/Potassium hydroxide/Periodic Acid-Schiff stain (PAT/KOH/PAS) and Mild Oxydation-Thionin Schiff/Periodic acid Borohydride/Potassium hydroxide/Periodic Acid-Schiff stain (MOT/PBT/KOH/PAS), (2) Immuno-histochemical stains with biotinylated lectins, including Dolichos Biflorus Aggulutinin (DBA), Griffonia Simplicifolia I (GS-I), Ulex Europaeus Agglutinin (UEA-I), Peanut Aggulutinin (PNA), Concanavalin A (Con A), Ricinus Communis Agglutinin I (RCA 120), Soybean Aggulutinin (SBA), and wheat germ aggulutinin (WGA). In the modified Schiff stains, tumor cells contained o-acetylated sialates or frequently lost the ability to produce sialates, while the normal endocervical cells produced predominantly non-acetylated sialates. Abrupt transition was also confirmed by these stainings. Based on the lectin study, the frequency of the positive reaction to PNA and Con A was statistically high in EA (p<0.005). The nuclear stratification index (NSI) was calculated positive in the cancerous glands for histochemical reactions, and the NSI of EA's was higher than that of normal cervices (P <0.05). These histochemical reactions were statistically significance and indicated both abrupt transition and NSI as important atypisms for cancer diagnosis.},
 pages = {75--84},
 title = {Histochemical and morphometric studies of endocervical type adenocarcinoma in the cervix uteri},
 volume = {36},
 year = {1988}
}