@article{oai:niigata-u.repo.nii.ac.jp:00006621,
 author = {Tanigaki, Noriko and Ito, Masaaki and Ando, Hideya and Kitano, Yukio and Hashimoto, Akira and Masamoto, Yukimitsu},
 issue = {4},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Dec},
 note = {Calcium regulation of murine hair and hair follicle cells grown in culture was electron-microscopically studied and the quantitative analysis of both growth and differentiation was performed. Some (47%) of the cultured cells were immature and others (53%) were already differentiated on Day 1 of the culture. In low calcium (0.03 mM) media, all of the cultured cells were immature on Day 3, and 90% of the cells were differentiated on Day 6. Twenty-five % of the latter cells were identified as inner root sheath cells, 55% as cortical or outer root sheath cells and 10% as medulla cells. The features of the cultured cells in high calcium (1.0 mM) media were different; although all of the cells were immature on Day 3, keratinization occurred in 27% of the cultured cells on Day 6. These keratinized cells were analogous in ultrastructure to those of in vivo keratinized inner root sheath or cortical cells. Twenty-two % of the cells showed degeneration. Most of the cells cultured in media with calcium levels lower than 0.03 mM showed ultrastructural features of degeneration on Day 6. These findings suggest that calcium plays an important role in the regulation of growth, differentiation and keratinization of cultured hair and hair follicle cells.},
 pages = {187--192},
 title = {Calcium Regulation of Growth, Differentiation and Keratinization of Cultured Cells from Murine Hair and Hair Follicles : An Ultrastructural Study},
 volume = {38},
 year = {1990}
}