@article{oai:niigata-u.repo.nii.ac.jp:00006610,
 author = {Ochi, Hiroshi and Morita, Ikuo and Murota, Sei-itsu},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Mar},
 note = {Among various arachidonic acid metabolites examined, only 15 (S)-hydroperoxy-5, 8, 11, 13-eicosatetraenoic acid (15-HPETE) caused severe injury to bovine endothelial cells in culture. This cellular injury was time- and dose-dependent. When compared with tertiary-butylhydroperoxide (t-BuOOH) and hydrogen peroxide (H_2O_2), it revealed that the injury caused by 15-HPETE was essentially distinct from those by tBuOOH and H_2O_2 in such respects as temperature dependency and albumin effect. When the endothelial monolayers were exposed to 15-HPETE, prostacyclin (PGI_2) production was markedly suppressed. However, there were discrepancies in time course and dose dependence between 15-HPETE-induced cellular injury and 15-HPETE-induced inhibition of PGI_2 synthesis. In addition, the injurious activity of 15-HPETE was not enhanced even when endothelial monolayers were pretreated with inhibitors of PGI_2 production, suggesting that endogenous PGI_2 does not play any cytoprocective role in endothelial cells at least against such a lipid peroxide as 15-HPETE.},
 pages = {21--29},
 title = {Endothelial Cell Injury caused by 15-HPETE, a Lipoxygenase Product of Arachidonic acid},
 volume = {38(Supplement)},
 year = {1990}
}