@article{oai:niigata-u.repo.nii.ac.jp:00006600,
 author = {Kashima, Yuji},
 issue = {2},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Jun},
 note = {A new cultured cell line (ACL-15) was established from a transplantable rat colon cancer strain induced by 1, 2-dimethyl hydrazine (DMH). Histologically, the primary colonic tumor was a poorly differentiated adenocarcinoma; all transplanted tumors showed the same histologic features. The doubling time of the ACL-15 cell line population was 24 h, and the saturation density was 5.6 x 10^5 cells/cm^2 in culture. The chromosome number and DNA content of the ACL-15 cells were near tetraploid. ACL-15 cells frequently metastasized to the liver and lungs in vivo. Thirty percent of those rats inoculated with ACL-15 cells into the subserosa of the cecum and all rats which received intrasplenic inoculations of ACL-15 cells developed hepatic metastases. All rats injected with ACL-15 into subcutis and into the tail vein developed multiple pulmonary metastases. This newly established cell line, ACL15, can serve for a model of metastatic colonic cancer.},
 pages = {75--81},
 title = {The Establishment of a Rat Colon Cancer Cell Line, ACL-15, with an Analysis of Its Metastatic Properties},
 volume = {39},
 year = {1991}
}