@article{oai:niigata-u.repo.nii.ac.jp:00006577,
 author = {Kawachi, Hiroshi},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Mar},
 note = {Two kinds of monoclonal antibodies (mAbs) capable of inducing massive proteinuria in rats were produced in BALB/c mice with collagenase-treated Wistar rat glomeruli. The first mAb (mAb 5-1-6) reacts with the surface of glomerular epithelial cell foot processes, mainly to slit diaphragms. Protein excretion was initiated immediately following an injection of 2 mg mAb 5-1-6, and reached an average value of 138.5 mg/24 h on day 5. No histological abnormalities were noted except for partial retraction of epithelial foot processes at the peak of proteinuria. The minimum dose of mAb 5-1-6 required to induce proteinuria is 0.125 mg as injected dose. This dose corresponds to 12.8 μg as the amount of kidney binding antibody 1 h after injection, and 0.34 μg at 5 days. The second mAb (mAb 1-22-3) is capable of inducing not only proteinuria but also morphological changes in the mesangial region. mAb 1-22-3 binds in vitro to the limited mesangial cell surface facing endothelial cells. Reactivity of mAb 1-22-3 toward any other site in the kidney except the mesangial cell surface could not be detected. These mAbs may possibly serve as a means for clarifying the mechanism regulating the macromolecule permeability of glomerular capillary wall.},
 pages = {47--52},
 title = {Proteinuria-Inducing Monoclonal Antibodies},
 volume = {39(Supplement)},
 year = {1991}
}