@article{oai:niigata-u.repo.nii.ac.jp:00006548,
 author = {Sato, Yoshinobu and Fukuda, Minoru},
 issue = {4},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Dec},
 note = {A trial administration of OK-432 by transarterial embolization (TAE) and intra-operative local injection (OLI) was performed to treat the liver metastasis of gastric cancer. The liver metastases decreased in size as assessed by abdominal CT scan. The peripheral level of DR^+ CD3^- monocytes (antigen-presenting cell) were increased on the day following OK-432 administration, and the increase in CD4^+ T lymphocytes (helper T cell) was prominent from 3 to 5 days afterward. Furthermore, the CD8^+ T lymphocyte (cytotxic T cell) level increased from 5 to 7 days after OK-432 treatment. The increase in the number of these lymphocytes after OLI with OK-432 was more prominent than that after TAE with OK--432. The concentration of DR^+CD4^+ T lymphocytes changed little, however, DR^+CD8^+ T lymphocytes increasing remarkably after TAE and OLI. NK activities decreased significantly on the day following TAE and abdominal operation, but increased afterward to at least pretreatment levels. On two-color flow cytometry using LEU 7 and LEU 11, which recognize NK surface antigens, each subpopulation of NK cells showed similar changes. However, LEU 11-positive cells comprised the majority of the NK cells. The kinetics of the NK subpopulation did not always correspond to that of NK activity, which may be due to the participation of neutrophils induced\nby the liver biopsy. These results suggest that the local administration of OK-432 by TAE and OLI activates cell mediated immunity by stimulating antigen-presenting cells and helper and cytotoxic T cells, and may therefore be used to treat liver metastasis.},
 pages = {151--156},
 title = {Immunological Monitoring of the Peripheral Blood Mononuclear Cell by Two-Color Flow Cytometry after Local Administration of OK-432 in a Patient with Gastric Cancer Displaying Multiple Liver Metastasis},
 volume = {40},
 year = {1992}
}