@article{oai:niigata-u.repo.nii.ac.jp:00006487,
 author = {Matsumoto, Mitsuo and Igarashi, Masahiko and Tominaga, Makoto and Daimon, Makoto and Fukase, Norio and Sugiyama, Kazuhiko and Ogawa, Atsushi and Yamatani, Keiichi and Sasaki, Hideo},
 issue = {4},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Dec},
 note = {To elucidate the occurrence of insulin resistance in metabolic ketoacidosis, we examined the hepatic glucose output (HGO) and glucose disappearance rate (Gd) by a euglycemic insulin clamp study on male Wistar rats under experimental metabolic ketosis and acidosis. Metabolic ketosis and acidosis were induced by the continuous intravenous infusion of either 3-hydroxybutyrate or ammonium chloride, respectively. Arterial blood gas analysis was performed by use of a blood gas analyzer. Insulin was infused at a rate of 3 mU/kg/min (low dose) or 20 mU/kg/min (high dose) during the glucose clamp course. Insulin sensitivity was evaluated by the mean of steady state glucose infusion rate (GIR) during the last 20 min. HGO and Gd were measured using 3- [_＾3H] -glucose. Steady state GIR of acidotic rats was 2.65±0.25 mg/kg/min with low dose insulin and 7.22±0.42 mg/kg/min with high dose insulin. Thus, steady state GIR in both condition was significantly lower than those of the control rats. Steady state HGO of acidotic rats was not suppressed under low dose insulin infusion, while HGO of the normal control rats was completely suppressed. On the other hand, neither the GIR and HGO of the ketotic rats differed from those of the control. Our results suggest that metabolic acidosis rather than ketosis is responsible for insulin resistance in metabolic ketoacidosis.},
 pages = {151--158},
 title = {Acidosis rather than Ketosis Reduces Insulin Action during Metabolic Ketoacidosis : An Investigation by Glucose Clamp Study in the Rat},
 volume = {42},
 year = {1994}
}