{"_buckets": {"deposit": "28fa4b64-7299-47d6-97b8-fffea68044a1"}, "_deposit": {"id": "6477", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "6477"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00006477"}, "item_7_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "1995-03", "bibliographicIssueDateType": "Issued"}, "bibliographicIssueNumber": "1", "bibliographicPageEnd": "10", "bibliographicPageStart": "1", "bibliographicVolumeNumber": "43", "bibliographic_titles": [{"bibliographic_title": "Acta medica et biologica"}, {"bibliographic_title": "Acta medica et biologica", "bibliographic_titleLang": "en"}]}]}, "item_7_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "The effects of Ca^\u003c2+\u003e, Ba^\u003c2+\u003e, Mg^\u003c2+\u003e and Zn^\u003c2+\u003e on the activity of heteromeric N-methyl-D-aspartate (NMDA) receptor channels (\u03b51/\u03b61, \u03b52/\u03b61, \u03b53/\u03b61 and \u03b54/\u03b61) expressed in Xenopus laevis oocytes were examined using the whole-cell clamp technique. The whole-cell current in response to 10\u03bcM glutamate plus 10\u03bcM glycine consisted of two phases: an initial spikelike phase and a subsequent sustained phase. Profiles of the sustained phase were almost a plateau in oocytes expressing the \u03b52/\u03b61, \u03b53/\u03b61 and \u03b54/\u03b61 channels and in one third of those expressing the \u03b51/\u03b61 channel. In two thirds of the oocytes expressing the \u03b51/\u03b61 channel, the sustained phase gradually increased. The initial spikelike phase was enhanced as the extracellular Ca^\u003c2+\u003e concentration increased, but the enhancement was suppressed by an intracellular injection of ethyleneglycol-bis(\u03b2-aminoethyl ether)-N,N,N\u0027N\u0027-tetraacetic acid (EGTA). Therefore, this spike-like current may be induced by intracellular Ca^\u003c2+\u003e-dependent processes: Ca^\u003c2+\u003e-dependent CI^- -conductance. Extracellular Ca^\u003c2+\u003e suppressed the flat sustained phase in oocytes expressing the \u03b51/\u03b61, \u03b53/\u03b61 and \u03b54/\u03b61 channels in a concentration-dependent manner. However, no comparable effect was observed in oocytes expressing the \u03b52/\u03b61 channel in which the whole-cell current was unchanged at 0.2, 0.6 and 2.0 mM extracellular Ca^\u003c2+\u003e. This sustained phase was unchanged by EGTA injection into oocytes expressing the \u03b51/\u03b61, \u03b52/\u03b61and \u03b53/\u03b61 channels and only partially inhibited in oocytes expressing the \u03b54/\u03b61 channel. Ba^\u003c2+\u003e also inhibited the flat sustained phase in oocytes expressing the \u03b51/\u03b61, \u03b53/\u03b61 and \u03b54/\u03b61 channels, but not those expressing the \u03b52/\u03b61 channel. Mg^\u003c2+\u003e and Zn^\u003c2+\u003e suppressed the sustained current in a concentration-dependent manner in oocytes expressing every heteromeric channel. However, the \u03b53/\u03b61 and \u03b54/\u03b61 channels were more resistant to Mg^\u003c2+\u003e than the \u03b51/\u03b61 and \u03b52/\u03b61 channels. The inhibitory effects of Zn^\u003c2+\u003e on heteromeric NMDA channels were similar to those of Mg^\u003c2+\u003e, except for the \u03b51/\u03b61 channel. The gradually increasing current observed in oocytes expressing the \u03b51/\u03b61 channel was very sensitive to Zn^\u003c2+\u003e, and the sustained phase showed a plateau in the presence of 1\u03bcM Zn^\u003c2+\u003e. These diverse sensitivities of heteromeric channels to divalent cations may depend on properties of the \u03b5 subunits. The characterization of these heteromeric NMDA channels based on their sensitivities to divalent cations, which is demonstrated in this paper, may be useful in understanding the physiological significance of the molecular diversity of the NMDA receptor channels.", "subitem_description_type": "Abstract"}]}, "item_7_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "Niigata University School of Medicine"}]}, "item_7_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "publisher"}]}, "item_7_source_id_11": {"attribute_name": "\u66f8\u8a8c\u30ec\u30b3\u30fc\u30c9ID", "attribute_value_mlt": [{"subitem_source_identifier": "AA00508361", "subitem_source_identifier_type": "NCID"}]}, "item_7_source_id_9": {"attribute_name": "ISSN", "attribute_value_mlt": [{"subitem_source_identifier": "05677734", "subitem_source_identifier_type": "ISSN"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Masaki, Hisashi"}], "nameIdentifiers": [{"nameIdentifier": "53119", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-06"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "43(1)_1-10.pdf", "filesize": [{"value": "1.4 MB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 1400000.0, "url": {"label": "43(1)_1-10.pdf", "url": "https://niigata-u.repo.nii.ac.jp/record/6477/files/43(1)_1-10.pdf"}, "version_id": "fa2f00f4-9377-4afb-8514-731e6820629c"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "departmental bulletin paper", "resourceuri": "http://purl.org/coar/resource_type/c_6501"}]}, "item_title": "Effects of Divalent Cations on Heteromeric NMDA Receptor Channels Expressed in Xenopus Oocytes", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Effects of Divalent Cations on Heteromeric NMDA Receptor Channels Expressed in Xenopus Oocytes"}, {"subitem_title": "Effects of Divalent Cations on Heteromeric NMDA Receptor Channels Expressed in Xenopus Oocytes", "subitem_title_language": "en"}]}, "item_type_id": "7", "owner": "1", "path": ["453/456", "471/537/568/624"], "permalink_uri": "http://hdl.handle.net/10191/33191", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2015-08-31"}, "publish_date": "2015-08-31", "publish_status": "0", "recid": "6477", "relation": {}, "relation_version_is_last": true, "title": ["Effects of Divalent Cations on Heteromeric NMDA Receptor Channels Expressed in Xenopus Oocytes"], "weko_shared_id": null}