@article{oai:niigata-u.repo.nii.ac.jp:00006462, author = {Kamei, Kiyomitsu and Matsuda, Hiroto and Yazawa, Yoshimitsu and Aizawa, Yoshifusa and Shibata, Akira}, issue = {3}, journal = {Acta medica et biologica, Acta medica et biologica}, month = {Sep}, note = {In a previous study, the authors observed an increase in the echo from the myocardium and endocardium, calling this "enhanced echocardiography." This experiment was conducted to confirm this finding and the mechanism of the enhancement. Six adult mongrel dogs had their chests opened under general anesthesia, and a minitransducer was sewed onto the epicardium which was perfused by the diagonal branch of the left coronary artery. After confirmation of stabilization of the echocardiogram from the endocardium by the minitransducer, indocyanin green (ICG) dissolved in normal saline was injected into the left ventricle and the effect on the endocardial echo was compared. The effect of repeated administrations of ICG solution on the endocardial echo was examined. At the end of the study, the heart was removed at the timing of the peak enhancement by ICG and the myocardium was examined microscopically. Immediately after the administration of ICG solution, linear echoes were observed in the myocardium and accentuation of the endocardial echo was observed for 3-5 min (average 4.2 min). The echo returned to the basal level within 15 min. By repeated administration of ICG in three of the six dogs, the accentuation of the endocardial echo was observed for a longer time, the average being 20 min. Microscopic examination revealed no ICG deposit in the myocardium nor microbubbles; instead, massive rouleaus of red blood cells were found in the small vessels.}, pages = {135--138}, title = {Enhanced Echocardiography with Indocyanin-Green Solution of Cardiac Wall}, volume = {43}, year = {1995} }