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  1. 0 資料タイプ別
  2. 03 紀要論文
  1. 250 大学院医歯学総合研究科(医)
  2. 20 紀要
  3. 01 Acta medica et biologica
  4. Vol.43 No.3

Effects of Depogen on Various Isozymes of Cyclic Nucleotide Phosphodiesterase Isolated from Porcine Aorta

http://hdl.handle.net/10191/33208
http://hdl.handle.net/10191/33208
93ba0a50-7505-4c76-9c91-9b6ec9e8222a
名前 / ファイル ライセンス アクション
43(3)_127-134.pdf 43(3)_127-134.pdf (833.4 kB)
Item type 紀要論文 / Departmental Bulletin Paper(1)
公開日 2015-08-31
タイトル
タイトル Effects of Depogen on Various Isozymes of Cyclic Nucleotide Phosphodiesterase Isolated from Porcine Aorta
タイトル
言語 en
タイトル Effects of Depogen on Various Isozymes of Cyclic Nucleotide Phosphodiesterase Isolated from Porcine Aorta
言語
言語 eng
キーワード
主題Scheme Other
主題 depogen
キーワード
主題Scheme Other
主題 PDE
キーワード
主題Scheme Other
主題 vascular smooth muscle
キーワード
主題Scheme Other
主題 cAMP
キーワード
主題Scheme Other
主題 cGMP
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ departmental bulletin paper
著者 Cai, Ji-Qun

× Cai, Ji-Qun

WEKO 53044

Cai, Ji-Qun

Search repository
Yoshida, Yutaka

× Yoshida, Yutaka

WEKO 53045

Yoshida, Yutaka

Search repository
Imai, Shoichi

× Imai, Shoichi

WEKO 53046

Imai, Shoichi

Search repository
抄録
内容記述タイプ Abstract
内容記述 Cyclic nucleotide phosphodiesterase (PDE) isozymes were separated from the soluble fraction of porcine aortic smooth muscle by DEAE-Sepharose Fast Flow ion exchange chromatography, and the effects of depogen (a theophilline-7 -acetice acid salt of drotaverine) on the isozymes were investigated in comparison with those of various selective or nonselective PDE inhibitors. Five PDE isozymes (Types I-V) were identified on the basis of their regulatory and kinetic properties and their sensitivities to selective PDE inhibitors. Type I (calmodulin-stimulated) preferentially hydrolyzed cGMP. The presence of Type II was demonstrated by a marked stimulation of activity by cGMP. Because of an insufficient amount of the isozyme and the unavailability of specific inhibitors, no further study of the Type II PDE was conducted. The Type III (cGMP-inhibited) and Type IV(cAMP-specific) isozymes preferentially hydrolyzed cAMP. The Type V PDE (cGMP-specific) hydrolyzed cGMP with a high selectivity. Depogen inhibited Types I, III and V PDE isozymes with potencies much weaker than selective inhibitors of each PDE isozyme as well as a nonselective PDE inhibitor, 3-isobutyl- 1-methylxanthine. In contrast, the inhibitory action of depogen was most potent toward Type IV PDE (IC_<50> =17μM), being more potent than that of RO20-1724, a Type IV-specific inhibitor (IC_<50>=32μM). Theophilline-7-acetic acid produces only a minimal inhibition while the inhibitory activities of drotaverine were very much like those of depogen. These results suggest that depogen is a selective inhibitor of Type IV PDE isozyme and that the inhibitory activity of depogen is mainly attributable to drotaverine.
書誌情報 Acta medica et biologica
en : Acta medica et biologica

巻 43, 号 3, p. 127-134, 発行日 1995-09
出版者
出版者 Niigata University School of Medicine
ISSN
収録物識別子タイプ ISSN
収録物識別子 05677734
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA00508361
著者版フラグ
値 publisher
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