@article{oai:niigata-u.repo.nii.ac.jp:00006434, author = {Nakagawa, Satoru and Watanabe, Hidenobu and Ajioka, Yoichi and Nishikura, Ken and Hitomi, Jiro and Hatakeyama, Katsuyoshi}, issue = {2}, journal = {Acta medica et biologica, Acta medica et biologica}, month = {Jun}, note = {Alterations in p53 were analyzed by immunochemistry in 131 lesions from 108 patients and by polymerase chain reaction single-strand conformation polymorphism(PCR-SSCP) in 69 tumors from 69 patients with esophageal squamous cell carcinomas, to elucidate the correlation between protein overexpression and mutation, and between p53 alteration and clinicopathological factors. The p53 gene-product was overexpressed in 83 (63%) of the 131, and mutations were detected in 24 (35%) of the 69 tumors examined. The mutations were predominantly located at exons 5 (58%) and 7 (27%), and detected in 13 (31%) of 42 overexpressing tumors, in none of 4 sporadic tumors and in 8 (40%) of 20 negative tumors. The p53 alteration did not correlate with the clinicopathological factors, including pTNM categories, stage, depth of invasion, and size of intramucosal carcinomas. These results indicate that p53 protein overexpression and gene mutation may occur during an early stage of esophageal carcinogenesis and have no impact on clinicopathological factors. The higher frequency of p53 overexpression compared to mutation suggests that p53 accumulation can occur by other mechanisms besides p53 mutation.}, pages = {63--69}, title = {Archival Analysis of p53 Protein Overexpression and Genetic Mutation in Esophageal Squamous Cell Carcinoma}, volume = {44}, year = {1996} }