@article{oai:niigata-u.repo.nii.ac.jp:00006397,
 author = {Sato, Nobuaki and Kanda, Tatsuo and Koyama, Yu and Kayama, Seiji and Ohkawa, Akira and Oyamatsu, Manabu and Hayashi, Mitsuhiro and Sato, Tomoi and Hatakeyama, Katsuyoshi},
 issue = {3},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Sep},
 note = {Background: Insulin-like growth factor-I (IGF-I) is a polypeptide hormone that is an important mediator of cellular growth, replication, and differentiation. IGF-I provides a major stimulus for enterocyte proliferation and the absorptive function. The aim of this study was to determine the effect of the intraluminal administration of IGF-I on the gastrointestinal tracts of rats with methotrexate (MTX)-induced enterocolitis. Study design: Male Sprague-Dawley rats weighing 140 to 205 g were intraperitoneally implanted with osmotic minipumps that delivered either 0.9% NaCl (saline group; n=8) or 10 nM IGF-I (IGF group; n= 9) to the ileal lumen through a short silastic catheter at a rate of 1μL/h. Rats received 20 mg/kg intraperitoneal MTX at the time of catheter insertion. Forty-eight hours after the MTX infusion, the small intestine was excised to measure mucosal protein and DNA content in jejunal and ileal segments. Additional measurements during the study period included changes in body weight and urinary nitrogen loss. Results: No significant difference in plasma IGF-I levels was seen between the groups. The saline group lost more body weight than the IGF group (p<0.05). In the IGF group, neither protein nor DNA content of the jejunum differed from those measured in the ileum. However, the ratio of the ileal to the jejunal protein content was significantly higher in the IGF group than in the saline group. Conclusion: Intraluminal administration of IGF-I may play a role in changing the distribution of gut mucosal proteins, in this rat model of MTX-induced enterocolitis.},
 pages = {115--119},
 title = {Effect of Intraluminal Administration of Insulin-like Growth Factor-I on Rats with Methotrexate-induced Enterocolitis},
 volume = {45},
 year = {1997}
}