@article{oai:niigata-u.repo.nii.ac.jp:00006363,
 author = {Maruyama, Yoshie and Yoshizawa, Hirohisa and Arakawa, Masaaki},
 issue = {4},
 journal = {Acta medica et biologica, Acta medica et biologica},
 month = {Dec},
 note = {We previously demonstrated that tumorspecific effector cells could be generated from tumordraining lymph node cells by in vitro sequential activation with anti-CD3 mAb and IL-2. In the present study, we further examined cellular interactions and mechanisms of the antitumor effect. Tumor-draining lymph node cells (TDLN) of a weakly immunogenic fibrosarcoma, MCA 205, were activated by the anti-CD3/IL-2 method and transferred into the peritoneal cavities of mice bearing 5-day established peritoneal dissemination of MCA 205 tumor cells. Intraperitoneal injection of anti-CD3/IL-2-activated TDLN significantly prolonged the survival period, and 80% of treated mice remained tumor free for more than 80 days. In vivo depletion of host macrophages with GdCI_3 clearly abrogated the antitumor effect, while the elimination of host T cells with sublethal irradiation (500 R) did not, indicating that host macrophages are indispensable in the antitumor effect. In vitro cytotoxicity and cell proliferation were further examined in detail to determine the commitment of macrophages and mediator cytokines. Although anti-CD3/IL-2 activated cells mediated minimal cytotoxicity in a 4-hr ^<51>Cr releasing assay, tumor specific cytolytic action was detected in an 18-hr assay with coexisting peritoneal cells. The results suggest that peritoneal macrophages play an indispensable role in this treatment effect and that T cell-macrophage interactions were mediated by IFN-γ, the importance of which was exhibited in the early phase of the tumor-effector-macrophage contact.},
 pages = {121--131},
 title = {Mechanism of Antitumor Effect Mediated by In Vitro Activated Tumor-draining Lymph Node Cells},
 volume = {46},
 year = {1998}
}