WEKO3
アイテム
Mechanism of Antitumor Effect Mediated by In Vitro Activated Tumor-draining Lymph Node Cells
http://hdl.handle.net/10191/33090
http://hdl.handle.net/10191/330906c31848b-999a-499a-88ac-58cdfee48aaa
| 名前 / ファイル | ライセンス | アクション |
|---|---|---|
46(4)_121-131.pdf (1.1 MB)
|
|
| Item type | 紀要論文 / Departmental Bulletin Paper(1) | |||||
|---|---|---|---|---|---|---|
| 公開日 | 2015-08-21 | |||||
| タイトル | ||||||
| タイトル | Mechanism of Antitumor Effect Mediated by In Vitro Activated Tumor-draining Lymph Node Cells | |||||
| タイトル | ||||||
| タイトル | Mechanism of Antitumor Effect Mediated by In Vitro Activated Tumor-draining Lymph Node Cells | |||||
| 言語 | en | |||||
| 言語 | ||||||
| 言語 | eng | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | adoptive immunotherapy | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | peritoneal cavity | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | macrophages | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | GdCl_3 | |||||
| キーワード | ||||||
| 主題Scheme | Other | |||||
| 主題 | IFN-γ | |||||
| 資源タイプ | ||||||
| 資源 | http://purl.org/coar/resource_type/c_6501 | |||||
| タイプ | departmental bulletin paper | |||||
| 著者 |
Maruyama, Yoshie
× Maruyama, Yoshie× Yoshizawa, Hirohisa× Arakawa, Masaaki |
|||||
| 抄録 | ||||||
| 内容記述タイプ | Abstract | |||||
| 内容記述 | We previously demonstrated that tumorspecific effector cells could be generated from tumordraining lymph node cells by in vitro sequential activation with anti-CD3 mAb and IL-2. In the present study, we further examined cellular interactions and mechanisms of the antitumor effect. Tumor-draining lymph node cells (TDLN) of a weakly immunogenic fibrosarcoma, MCA 205, were activated by the anti-CD3/IL-2 method and transferred into the peritoneal cavities of mice bearing 5-day established peritoneal dissemination of MCA 205 tumor cells. Intraperitoneal injection of anti-CD3/IL-2-activated TDLN significantly prolonged the survival period, and 80% of treated mice remained tumor free for more than 80 days. In vivo depletion of host macrophages with GdCI_3 clearly abrogated the antitumor effect, while the elimination of host T cells with sublethal irradiation (500 R) did not, indicating that host macrophages are indispensable in the antitumor effect. In vitro cytotoxicity and cell proliferation were further examined in detail to determine the commitment of macrophages and mediator cytokines. Although anti-CD3/IL-2 activated cells mediated minimal cytotoxicity in a 4-hr ^<51>Cr releasing assay, tumor specific cytolytic action was detected in an 18-hr assay with coexisting peritoneal cells. The results suggest that peritoneal macrophages play an indispensable role in this treatment effect and that T cell-macrophage interactions were mediated by IFN-γ, the importance of which was exhibited in the early phase of the tumor-effector-macrophage contact. | |||||
| 書誌情報 |
Acta medica et biologica en : Acta medica et biologica 巻 46, 号 4, p. 121-131, 発行日 1998-12 |
|||||
| 出版者 | ||||||
| 出版者 | Niigata University School of Medicine | |||||
| ISSN | ||||||
| 収録物識別子タイプ | ISSN | |||||
| 収録物識別子 | 05677734 | |||||
| 書誌レコードID | ||||||
| 収録物識別子タイプ | NCID | |||||
| 収録物識別子 | AA00508361 | |||||
| 著者版フラグ | ||||||
| 値 | publisher | |||||
