@misc{oai:niigata-u.repo.nii.ac.jp:00005989,
 author = {Ogawa, Kohei},
 month = {Mar},
 note = {Development of an effective, safe, and convenient method for gene delivery to the pancreas is a critical step toward gene therapy for pancreatic diseases. Therefore, we tested the possibility of applying the principle of hydrodynamic gene delivery for successful gene transfer to pancreas using rats as a model. The established procedure involves the insertion of a catheter into the superior mesenteric vein with temporary blood flow occlusion at the portal vein and hydrodynamic injection of DNA solution. We demonstrated that our procedure achieved efficient pancreas-specific gene expression that was 2,000-fold higher than that seen in the pancreas after the systemic hydrodynamic gene delivery. In addition, the level of gene expression achieved in the pancreas by the pancreas-specific gene delivery was comparable to the level in the liver achieved by a liver-specific hydrodynamic gene delivery. The optimal level of reporter gene expression in the pancreas requires an injection volume equivalent to 2.0% body weight with flow rate of 1 mL/s and plasmid DNA concentration at 5 mg/mL. With the exception of transient expansion of intercellular spaces and elevation of serum amylase levels, which recovered within 3 days, no permanent tissue damage was observed. These results suggest that pancreas-targeted hydrodynamic gene delivery is an effective and safe method for gene delivery to the pancreas and clinically applicable., 学位の種類: 博士（医学）. 報告番号: 甲第4391号. 学位記番号: 新大院博（医）甲第790号. 学位授与年月日: 平成30年3月23日, Molecular Therapy Nucleic Acids. 2017. 9, 80-88., 新大院博（医）甲第790号},
 title = {Efficacy and Safety of Pancreas-Targeted Hydrodynamic Gene Delivery in Rats},
 year = {2018}
}