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  1. 0 資料タイプ別
  2. 02 学位論文
  1. 250 大学院医歯学総合研究科(医)
  2. 60 博士学位論文
  3. 10 博士学位論文

Novel kinase fusion transcripts found in endometrial cancer.

http://hdl.handle.net/10191/47585
http://hdl.handle.net/10191/47585
aacf0d68-e0cf-4e53-9322-284d26d3702c
名前 / ファイル ライセンス アクション
h28nmk732.pdf 本文 (1.1 MB)
h28nmk732_a.pdf 要旨 (193.1 kB)
Item type 学位論文 / Thesis or Dissertation(1)
公開日 2018-09-11
タイトル
タイトル Novel kinase fusion transcripts found in endometrial cancer.
タイトル
言語 en
タイトル Novel kinase fusion transcripts found in endometrial cancer.
言語
言語 eng
資源タイプ
資源 http://purl.org/coar/resource_type/c_46ec
タイプ thesis
その他のタイトル
その他のタイトル 子宮体癌における新規キナーゼ融合遺伝子の同定
著者 Tamura, Ryo

× Tamura, Ryo

WEKO 50967

Tamura, Ryo

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著者別名
識別子 50968
識別子Scheme WEKO
姓名 田村, 亮
抄録
内容記述タイプ Abstract
内容記述 Recent advances in RNA-sequencing technology have enabled the discovery of gene fusion transcripts in the transcriptome of cancer cells. However, it remains difficult to differentiate the therapeutically targetable fusions from passenger events. We have analyzed RNA-sequencing data and DNA copy number data from 25 endometrial cancer cell lines to identify potential therapeutically targetable fusion transcripts, and have identified 124 high-confidence fusion transcripts, of which 69% are associated with gene amplifications. As targetable fusion candidates, we focused on three in-frame kinase fusion transcripts that retain a kinase domain (CPQ-PRKDC, CAPZA2-MET, and VGLL4-PRKG1). We detected only CPQ-PRKDC fusion transcript in three of 122 primary endometrial cancer tissues. Cell proliferation of the fusion-positive cell line was inhibited by knocking down the expression of wild-type PRKDC but not by blocking the CPQ-PRKDC fusion transcript expression. Quantitative real-time RT-PCR demonstrated that the expression of the CPQ-PRKDC fusion transcript was significantly lower than that of wild-type PRKDC, corresponding to a low transcript allele fraction of this fusion, based on RNA-sequencing read counts. In endometrial cancers, the CPQ-PRKDC fusion transcript may be a passenger aberration related to gene amplification. Our findings suggest that transcript allele fraction is a useful predictor to find bona-fide therapeutic-targetable fusion transcripts.
内容記述
内容記述タイプ Other
内容記述 学位の種類: 博士(医学). 報告番号: 甲第4254号. 学位記番号: 新大院博(医)甲第732号. 学位授与年月日: 平成29年3月23日
内容記述
内容記述タイプ Other
内容記述 Scientific Reports 5, Article number: 18657 (2015)
書誌情報 発行日 2017-03-23
出版者
出版者 新潟大学
DOI
識別子タイプ DOI
関連識別子 info:doi/10.1038/srep18657
著者版フラグ
値 ETD
学位名
学位名 博士(医学)
学位授与機関
学位授与機関名 新潟大学
学位授与年月日
学位授与年月日 2017-03-23
学位授与番号
学位授与番号 13101甲第4254号
学位記番号
内容記述タイプ Other
内容記述 新大院博(医)甲第732号
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