@misc{oai:niigata-u.repo.nii.ac.jp:00005838,
 author = {Ando, Takashi},
 month = {Mar},
 note = {Intratumoural dihydrotestosterone (DHT) synthesis could be an explanation for castration resistance\nin prostate cancer (PC). By using liquid chromatography-mass spectrometry, we evaluated the\nintratumoral DHT synthesis from 5α-androstane-3β,17β-diol (3β-diol), which is inactive androgen metabolized from DHT. 3β-diol had biochemical potential to be converted to DHT via three metabolic pathways and could stimulate PC cell growth. Especially, 3β-diol was not only converted back to upstream androgens such as dehydroepiandrosterone (DHEA) or Δ5-androstenediol but also converted directly to DHT which is the main pathway from 3β-diol to DHT. Abiraterone had a significant influence on the metabolism of DHEA, epiandrosterone and 3β-diol, by the inhibition of the intratumoural 3β-hydroxysteroid dehydrogenase (3β-HSD) activities which is one of key catalysts in androgen metabolic pathway. The direct-conversion of 3β-diol to DHT was catalysed by 3β-HSD and abiraterone could inhibit this activity of 3β-HSD. These results suggest that PC had a mechanism of intratumoural androgen metabolism to return inactive androgen to active androgen and intratumoural DHT synthesis from 3β-diol is important as one of the mechanisms of castration resistance in PC. Additionally, the inhibition of intratumoural 3β-HSD activity could be a new approach to castration-resistant prostate\ncancer treatment., 学位の種類: 博士（医学）. 報告番号: 甲第4250号. 学位記番号: 新大院博（医）甲第728号. 学位授与年月日: 平成29年3月23日, Scientific Reports 6 Article number 32198,2016, 新大院博（医）甲第728号},
 title = {Dihydrotestosterone synthesis pathways from inactive androgen 5α-androstane-3β,17β-diol in prostate cancer cells: Inhibition of intratumoural 3β-hydroxysteroid dehydrogenase activities by abiraterone.},
 year = {2017}
}