@misc{oai:niigata-u.repo.nii.ac.jp:00005818,
 author = {Sakaue, Yuuki},
 month = {Sep},
 note = {Dental caries affects people of all ages and is a worldwide health concern. Streptococcus mutans is a major cariogenic bacterium because of its ability to form biofilm and induce an acidic environment. In this study, the antibacterial activities of magnolol and honokiol, the main constituents of the bark of\nmagnolia plants, toward planktonic cell and biofilm of S. mutans were examined and compared with those of chlorhexidine. The minimal inhibitory concentrations of magnolol, honokiol and chlorhexidine for S. mutans were 10, 10 and 0.25mg/mL, respectively. In addition, each agent showed bactericidal\nactivity against S.mutans planktonic cells and inhibited biofilmformationin a dose- andtime-dependent manner.Magnolol (50mg/mL) had greater bactericidal activity against S. mutans biofilm than honokiol (50mg/mL) and chlorhexidine (500mg/mL) at 5min after exposure, while all showed scant activity\nagainst biofilm at 30 s. Furthermore; chlorhexidine (0.5–500mg/mL) exhibited high cellular toxicity for the gingival epithelial cell lineCa9-22 at 1 hr,whereasmagnolol (50mg/mL) and honokiol (50mg/mL) did not. Thus; it was found thatmagnolol has antimicrobial activities against planktonic and biofilm cells of S. mutans. Magnolol may be a candidate for prevention and management of dental caries., 学位の種類: 博士（歯学）. 報告番号: 甲第4224号. 学位記番号: 新大院博（歯）甲第366号. 学位授与年月日: 平成28年9月20日, Microbiology and Immunology 2016; 60: 10–16, 新大院博（歯）甲第366号},
 title = {Anti-biofilm and bactericidal effects of magnolia bark-derived magnolol and honokiol on Streptococcus mutans},
 year = {2016}
}