{"created":"2021-03-01T06:09:37.511137+00:00","id":5755,"links":{},"metadata":{"_buckets":{"deposit":"ff912f4a-82f4-48da-8d67-f0f19e6e59d4"},"_deposit":{"id":"5755","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5755"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005755","sets":["432:433:434","453:455"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"日本人慢性歯周炎および関節リウマチ罹患成人におけるTNF-α遺伝子プロモーター領域メチル化の解析"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-23","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（歯学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"\"Background and Objective: Over-expression of tumor necrosis factor-alpha (TNF-a) plays a pathological role in chronic periodontitis (CP) and rheumatoid arthritis (RA), which might be regulated by the epigenetic mechanism. The aim of the present study was to evaluate whether there is a unique methylation profile of the TNF-a gene promoter in blood cells of individuals with CP and RA. Material and Methods: The study participants consisted of 30 Japanese adults with RA (RA group), 30 race-matched adults with CP only (CP group) and 30 race-matched healthy controls (H group). Genomic DNA isolated from peripheral blood was modified by sodium bisulfite and analyzed, by direct sequencing, to investigate DNA methylation of the TNF-a gene promoter region. The level of TNF-a produced in mononuclear cells stimulated with Porphyromonas gingivalis\\nlipopolysaccharide was determined using ELISA. Results: Twelve cytosine–guanine dinucleotide (CpG) motifs were identified in the TNF-a promoter fragment from  343 to +57 bp. The CP group showed a significantly higher methylation rate and frequency at  72 bp than the H group (p < 0.01). The RA group exhibited significantly higher methylation rates at seven CpG motifs ( 302,  163,  119,  72,  49,  38 and +10 bp), and significantly\\nhigher methylation frequencies at six CpG motifs ( 163,  119,  72, 49,  38 and +10 bp), than the H group (p < 0.01 for all comparisons). The levels of TNF-a produced were significantly different between individuals with and without methylation at  163 bp (p = 0.03). Conclusion: These results suggest that the hypermethylated status of CpG motifs in the TNF-a gene promoter in blood cells may be unique to Japanese adults with CP and RA.\"","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（歯学）. 報告番号: 甲第4148号. 学位記番号: 新大院博（歯）甲第346号. 学位授与年月日: 平成28年3月23日","subitem_description_type":"Other"},{"subitem_description":"Journal of Periodontal Research. 2016, 51(3), 350-358.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（歯）甲第346号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4148号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"50842","nameIdentifierScheme":"WEKO"}],"names":[{"name":"小島, 杏里"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1111/jre.12314","subitem_relation_type_select":"DOI"}}]},"item_6_rights_15":{"attribute_name":"権利","attribute_value_mlt":[{"subitem_rights":"【○!C】 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd."}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"none"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Kojima, Anri"}],"nameIdentifiers":[{"nameIdentifier":"50841","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h27ndk346_b.pdf","filesize":[{"value":"90.6 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要約","url":"https://niigata-u.repo.nii.ac.jp/record/5755/files/h27ndk346_b.pdf"},"version_id":"041ab6ab-a20c-47bd-b4b8-ba1259e64e34"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h27ndk346_a.pdf","filesize":[{"value":"223.9 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5755/files/h27ndk346_a.pdf"},"version_id":"ad7cfaaf-8795-4508-9262-4cede87ed77a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"DNA methylation","subitem_subject_scheme":"Other"},{"subitem_subject":"periodontitis","subitem_subject_scheme":"Other"},{"subitem_subject":"rheumatoid arthritis","subitem_subject_scheme":"Other"},{"subitem_subject":"tumor necrosis factor-alpha","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis"},{"subitem_title":"Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","434"],"pubdate":{"attribute_name":"公開日","attribute_value":"2018-09-11"},"publish_date":"2018-09-11","publish_status":"0","recid":"5755","relation_version_is_last":true,"title":["Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T04:07:10.836686+00:00"}