{"_buckets": {"deposit": "ff912f4a-82f4-48da-8d67-f0f19e6e59d4"}, "_deposit": {"id": "5755", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5755"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00005755", "sets": ["455", "434"]}, "item_6_alternative_title_1": {"attribute_name": "その他のタイトル", "attribute_value_mlt": [{"subitem_alternative_title": "日本人慢性歯周炎および関節リウマチ罹患成人におけるTNF-α遺伝子プロモーター領域メチル化の解析"}]}, "item_6_biblio_info_6": {"attribute_name": "書誌情報", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-03-23", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_6_date_granted_51": {"attribute_name": "学位授与年月日", "attribute_value_mlt": [{"subitem_dategranted": "2016-03-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "学位授与機関", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "新潟大学"}]}]}, "item_6_degree_name_48": {"attribute_name": "学位名", "attribute_value_mlt": [{"subitem_degreename": "博士（歯学）"}]}, "item_6_description_4": {"attribute_name": "抄録", "attribute_value_mlt": [{"subitem_description": "\"Background and Objective: Over-expression of tumor necrosis factor-alpha (TNF-a) plays a pathological role in chronic periodontitis (CP) and rheumatoid arthritis (RA), which might be regulated by the epigenetic mechanism. The aim of the present study was to evaluate whether there is a unique methylation profile of the TNF-a gene promoter in blood cells of individuals with CP and RA. Material and Methods: The study participants consisted of 30 Japanese adults with RA (RA group), 30 race-matched adults with CP only (CP group) and 30 race-matched healthy controls (H group). Genomic DNA isolated from peripheral blood was modified by sodium bisulfite and analyzed, by direct sequencing, to investigate DNA methylation of the TNF-a gene promoter region. The level of TNF-a produced in mononuclear cells stimulated with Porphyromonas gingivalis\\nlipopolysaccharide was determined using ELISA. Results: Twelve cytosine–guanine dinucleotide (CpG) motifs were identified in the TNF-a promoter fragment from  343 to +57 bp. The CP group showed a significantly higher methylation rate and frequency at  72 bp than the H group (p \u003c 0.01). The RA group exhibited significantly higher methylation rates at seven CpG motifs ( 302,  163,  119,  72,  49,  38 and +10 bp), and significantly\\nhigher methylation frequencies at six CpG motifs ( 163,  119,  72, 49,  38 and +10 bp), than the H group (p \u003c 0.01 for all comparisons). The levels of TNF-a produced were significantly different between individuals with and without methylation at  163 bp (p = 0.03). Conclusion: These results suggest that the hypermethylated status of CpG motifs in the TNF-a gene promoter in blood cells may be unique to Japanese adults with CP and RA.\"", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "内容記述", "attribute_value_mlt": [{"subitem_description": "学位の種類: 博士（歯学）. 報告番号: 甲第4148号. 学位記番号: 新大院博（歯）甲第346号. 学位授与年月日: 平成28年3月23日", "subitem_description_type": "Other"}, {"subitem_description": "Journal of Periodontal Research. 2016, 51(3), 350-358.", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "学位記番号", "attribute_value_mlt": [{"subitem_description": "新大院博（歯）甲第346号", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "学位授与番号", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101甲第4148号"}]}, "item_6_full_name_3": {"attribute_name": "著者別名", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "50842", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "小島, 杏里"}]}]}, "item_6_publisher_7": {"attribute_name": "出版者", "attribute_value_mlt": [{"subitem_publisher": "新潟大学"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1111/jre.12314", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "権利", "attribute_value_mlt": [{"subitem_rights": "【○!C】 2015 John Wiley \u0026 Sons A/S. Published by John Wiley \u0026 Sons Ltd."}]}, "item_6_select_19": {"attribute_name": "著者版フラグ", "attribute_value_mlt": [{"subitem_select_item": "none"}]}, "item_creator": {"attribute_name": "著者", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kojima, Anri"}], "nameIdentifiers": [{"nameIdentifier": "50841", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "ファイル情報", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "h27ndk346_b.pdf", "filesize": [{"value": "90.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 90600.0, "url": {"label": "要約", "url": "https://niigata-u.repo.nii.ac.jp/record/5755/files/h27ndk346_b.pdf"}, "version_id": "041ab6ab-a20c-47bd-b4b8-ba1259e64e34"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "h27ndk346_a.pdf", "filesize": [{"value": "223.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 223900.0, "url": {"label": "要旨", "url": "https://niigata-u.repo.nii.ac.jp/record/5755/files/h27ndk346_a.pdf"}, "version_id": "ad7cfaaf-8795-4508-9262-4cede87ed77a"}]}, "item_keyword": {"attribute_name": "キーワード", "attribute_value_mlt": [{"subitem_subject": "DNA methylation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "periodontitis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rheumatoid arthritis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tumor necrosis factor-alpha", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "言語", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "資源タイプ", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis", "item_titles": {"attribute_name": "タイトル", "attribute_value_mlt": [{"subitem_title": "Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis"}, {"subitem_title": "Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["455", "434"], "permalink_uri": "http://hdl.handle.net/10191/42134", "pubdate": {"attribute_name": "公開日", "attribute_value": "2018-09-11"}, "publish_date": "2018-09-11", "publish_status": "0", "recid": "5755", "relation": {}, "relation_version_is_last": true, "title": ["Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis"], "weko_shared_id": 2}