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The aim of the present study was to evaluate whether there is a unique methylation profile of the TNF-a gene promoter in blood cells of individuals with CP and RA. Material and Methods: The study participants consisted of 30 Japanese adults with RA (RA group), 30 race-matched adults with CP only (CP group) and 30 race-matched healthy controls (H group). Genomic DNA isolated from peripheral blood was modified by sodium bisulfite and analyzed, by direct sequencing, to investigate DNA methylation of the TNF-a gene promoter region. The level of TNF-a produced in mononuclear cells stimulated with Porphyromonas gingivalis\\nlipopolysaccharide was determined using ELISA. Results: Twelve cytosine–guanine dinucleotide (CpG) motifs were identified in the TNF-a promoter fragment from 343 to +57 bp. The CP group showed a significantly higher methylation rate and frequency at 72 bp than the H group (p \u003c 0.01). The RA group exhibited significantly higher methylation rates at seven CpG motifs ( 302, 163, 119, 72, 49, 38 and +10 bp), and significantly\\nhigher methylation frequencies at six CpG motifs ( 163, 119, 72, 49, 38 and +10 bp), than the H group (p \u003c 0.01 for all comparisons). The levels of TNF-a produced were significantly different between individuals with and without methylation at 163 bp (p = 0.03). 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Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis
http://hdl.handle.net/10191/42134
http://hdl.handle.net/10191/42134926465a2-9d22-43d8-b3a1-3f310baa2868
名前 / ファイル | ライセンス | アクション |
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Item type | 学位論文 / Thesis or Dissertation(1) | |||||
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公開日 | 2018-09-11 | |||||
タイトル | ||||||
タイトル | Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis | |||||
タイトル | ||||||
言語 | en | |||||
タイトル | Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis | |||||
言語 | ||||||
言語 | eng | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | DNA methylation | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | periodontitis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | rheumatoid arthritis | |||||
キーワード | ||||||
主題Scheme | Other | |||||
主題 | tumor necrosis factor-alpha | |||||
資源タイプ | ||||||
資源 | http://purl.org/coar/resource_type/c_46ec | |||||
タイプ | thesis | |||||
その他のタイトル | ||||||
その他のタイトル | 日本人慢性歯周炎および関節リウマチ罹患成人におけるTNF-α遺伝子プロモーター領域メチル化の解析 | |||||
著者 |
Kojima, Anri
× Kojima, Anri |
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著者別名 | ||||||
識別子 | 50842 | |||||
識別子Scheme | WEKO | |||||
姓名 | 小島, 杏里 | |||||
抄録 | ||||||
内容記述タイプ | Abstract | |||||
内容記述 | "Background and Objective: Over-expression of tumor necrosis factor-alpha (TNF-a) plays a pathological role in chronic periodontitis (CP) and rheumatoid arthritis (RA), which might be regulated by the epigenetic mechanism. The aim of the present study was to evaluate whether there is a unique methylation profile of the TNF-a gene promoter in blood cells of individuals with CP and RA. Material and Methods: The study participants consisted of 30 Japanese adults with RA (RA group), 30 race-matched adults with CP only (CP group) and 30 race-matched healthy controls (H group). Genomic DNA isolated from peripheral blood was modified by sodium bisulfite and analyzed, by direct sequencing, to investigate DNA methylation of the TNF-a gene promoter region. The level of TNF-a produced in mononuclear cells stimulated with Porphyromonas gingivalis\nlipopolysaccharide was determined using ELISA. Results: Twelve cytosine–guanine dinucleotide (CpG) motifs were identified in the TNF-a promoter fragment from 343 to +57 bp. The CP group showed a significantly higher methylation rate and frequency at 72 bp than the H group (p < 0.01). The RA group exhibited significantly higher methylation rates at seven CpG motifs ( 302, 163, 119, 72, 49, 38 and +10 bp), and significantly\nhigher methylation frequencies at six CpG motifs ( 163, 119, 72, 49, 38 and +10 bp), than the H group (p < 0.01 for all comparisons). The levels of TNF-a produced were significantly different between individuals with and without methylation at 163 bp (p = 0.03). Conclusion: These results suggest that the hypermethylated status of CpG motifs in the TNF-a gene promoter in blood cells may be unique to Japanese adults with CP and RA." | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 学位の種類: 博士(歯学). 報告番号: 甲第4148号. 学位記番号: 新大院博(歯)甲第346号. 学位授与年月日: 平成28年3月23日 | |||||
内容記述 | ||||||
内容記述タイプ | Other | |||||
内容記述 | Journal of Periodontal Research. 2016, 51(3), 350-358. | |||||
書誌情報 | 発行日 2016-03-23 | |||||
出版者 | ||||||
出版者 | 新潟大学 | |||||
DOI | ||||||
識別子タイプ | DOI | |||||
関連識別子 | info:doi/10.1111/jre.12314 | |||||
権利 | ||||||
権利情報 | 【○!C】 2015 John Wiley & Sons A/S. Published by John Wiley & Sons Ltd. | |||||
著者版フラグ | ||||||
値 | none | |||||
学位名 | ||||||
学位名 | 博士(歯学) | |||||
学位授与機関 | ||||||
学位授与機関名 | 新潟大学 | |||||
学位授与年月日 | ||||||
学位授与年月日 | 2016-03-23 | |||||
学位授与番号 | ||||||
学位授与番号 | 13101甲第4148号 | |||||
学位記番号 | ||||||
内容記述タイプ | Other | |||||
内容記述 | 新大院博(歯)甲第346号 |