{"_buckets": {"deposit": "ff912f4a-82f4-48da-8d67-f0f19e6e59d4"}, "_deposit": {"id": "5755", "owners": [], "pid": {"revision_id": 0, "type": "depid", "value": "5755"}, "status": "published"}, "_oai": {"id": "oai:niigata-u.repo.nii.ac.jp:00005755"}, "item_6_alternative_title_1": {"attribute_name": "\u305d\u306e\u4ed6\u306e\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_alternative_title": "\u65e5\u672c\u4eba\u6162\u6027\u6b6f\u5468\u708e\u304a\u3088\u3073\u95a2\u7bc0\u30ea\u30a6\u30de\u30c1\u7f79\u60a3\u6210\u4eba\u306b\u304a\u3051\u308bTNF-\u03b1\u907a\u4f1d\u5b50\u30d7\u30ed\u30e2\u30fc\u30bf\u30fc\u9818\u57df\u30e1\u30c1\u30eb\u5316\u306e\u89e3\u6790"}]}, "item_6_biblio_info_6": {"attribute_name": "\u66f8\u8a8c\u60c5\u5831", "attribute_value_mlt": [{"bibliographicIssueDates": {"bibliographicIssueDate": "2016-03-23", "bibliographicIssueDateType": "Issued"}, "bibliographic_titles": [{}]}]}, "item_6_date_granted_51": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5", "attribute_value_mlt": [{"subitem_dategranted": "2016-03-23"}]}, "item_6_degree_grantor_49": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u6a5f\u95a2", "attribute_value_mlt": [{"subitem_degreegrantor": [{"subitem_degreegrantor_name": "\u65b0\u6f5f\u5927\u5b66"}]}]}, "item_6_degree_name_48": {"attribute_name": "\u5b66\u4f4d\u540d", "attribute_value_mlt": [{"subitem_degreename": "\u535a\u58eb\uff08\u6b6f\u5b66\uff09"}]}, "item_6_description_4": {"attribute_name": "\u6284\u9332", "attribute_value_mlt": [{"subitem_description": "\"Background and Objective: Over-expression of tumor necrosis factor-alpha (TNF-a) plays a pathological role in chronic periodontitis (CP) and rheumatoid arthritis (RA), which might be regulated by the epigenetic mechanism. The aim of the present study was to evaluate whether there is a unique methylation profile of the TNF-a gene promoter in blood cells of individuals with CP and RA. Material and Methods: The study participants consisted of 30 Japanese adults with RA (RA group), 30 race-matched adults with CP only (CP group) and 30 race-matched healthy controls (H group). Genomic DNA isolated from peripheral blood was modified by sodium bisulfite and analyzed, by direct sequencing, to investigate DNA methylation of the TNF-a gene promoter region. The level of TNF-a produced in mononuclear cells stimulated with Porphyromonas gingivalis\\nlipopolysaccharide was determined using ELISA. Results: Twelve cytosine\u2013guanine dinucleotide (CpG) motifs were identified in the TNF-a promoter fragment from  343 to +57 bp. The CP group showed a significantly higher methylation rate and frequency at  72 bp than the H group (p \u003c 0.01). The RA group exhibited significantly higher methylation rates at seven CpG motifs ( 302,  163,  119,  72,  49,  38 and +10 bp), and significantly\\nhigher methylation frequencies at six CpG motifs ( 163,  119,  72, 49,  38 and +10 bp), than the H group (p \u003c 0.01 for all comparisons). The levels of TNF-a produced were significantly different between individuals with and without methylation at  163 bp (p = 0.03). Conclusion: These results suggest that the hypermethylated status of CpG motifs in the TNF-a gene promoter in blood cells may be unique to Japanese adults with CP and RA.\"", "subitem_description_type": "Abstract"}]}, "item_6_description_5": {"attribute_name": "\u5185\u5bb9\u8a18\u8ff0", "attribute_value_mlt": [{"subitem_description": "\u5b66\u4f4d\u306e\u7a2e\u985e: \u535a\u58eb\uff08\u6b6f\u5b66\uff09. \u5831\u544a\u756a\u53f7: \u7532\u7b2c4148\u53f7. \u5b66\u4f4d\u8a18\u756a\u53f7: \u65b0\u5927\u9662\u535a\uff08\u6b6f\uff09\u7532\u7b2c346\u53f7. \u5b66\u4f4d\u6388\u4e0e\u5e74\u6708\u65e5: \u5e73\u621028\u5e743\u670823\u65e5", "subitem_description_type": "Other"}, {"subitem_description": "Journal of Periodontal Research. 2016, 51(3), 350-358.", "subitem_description_type": "Other"}]}, "item_6_description_53": {"attribute_name": "\u5b66\u4f4d\u8a18\u756a\u53f7", "attribute_value_mlt": [{"subitem_description": "\u65b0\u5927\u9662\u535a\uff08\u6b6f\uff09\u7532\u7b2c346\u53f7", "subitem_description_type": "Other"}]}, "item_6_dissertation_number_52": {"attribute_name": "\u5b66\u4f4d\u6388\u4e0e\u756a\u53f7", "attribute_value_mlt": [{"subitem_dissertationnumber": "13101\u7532\u7b2c4148\u53f7"}]}, "item_6_full_name_3": {"attribute_name": "\u8457\u8005\u5225\u540d", "attribute_value_mlt": [{"nameIdentifiers": [{"nameIdentifier": "50842", "nameIdentifierScheme": "WEKO"}], "names": [{"name": "\u5c0f\u5cf6, \u674f\u91cc"}]}]}, "item_6_publisher_7": {"attribute_name": "\u51fa\u7248\u8005", "attribute_value_mlt": [{"subitem_publisher": "\u65b0\u6f5f\u5927\u5b66"}]}, "item_6_relation_14": {"attribute_name": "DOI", "attribute_value_mlt": [{"subitem_relation_type_id": {"subitem_relation_type_id_text": "info:doi/10.1111/jre.12314", "subitem_relation_type_select": "DOI"}}]}, "item_6_rights_15": {"attribute_name": "\u6a29\u5229", "attribute_value_mlt": [{"subitem_rights": "\u3010\u25cb!C\u3011 2015 John Wiley \u0026 Sons A/S. Published by John Wiley \u0026 Sons Ltd."}]}, "item_6_select_19": {"attribute_name": "\u8457\u8005\u7248\u30d5\u30e9\u30b0", "attribute_value_mlt": [{"subitem_select_item": "none"}]}, "item_creator": {"attribute_name": "\u8457\u8005", "attribute_type": "creator", "attribute_value_mlt": [{"creatorNames": [{"creatorName": "Kojima, Anri"}], "nameIdentifiers": [{"nameIdentifier": "50841", "nameIdentifierScheme": "WEKO"}]}]}, "item_files": {"attribute_name": "\u30d5\u30a1\u30a4\u30eb\u60c5\u5831", "attribute_type": "file", "attribute_value_mlt": [{"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 0, "filename": "h27ndk346_b.pdf", "filesize": [{"value": "90.6 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 90600.0, "url": {"label": "\u8981\u7d04", "url": "https://niigata-u.repo.nii.ac.jp/record/5755/files/h27ndk346_b.pdf"}, "version_id": "041ab6ab-a20c-47bd-b4b8-ba1259e64e34"}, {"accessrole": "open_date", "date": [{"dateType": "Available", "dateValue": "2019-08-05"}], "displaytype": "detail", "download_preview_message": "", "file_order": 1, "filename": "h27ndk346_a.pdf", "filesize": [{"value": "223.9 kB"}], "format": "application/pdf", "future_date_message": "", "is_thumbnail": false, "licensetype": "license_free", "mimetype": "application/pdf", "size": 223900.0, "url": {"label": "\u8981\u65e8", "url": "https://niigata-u.repo.nii.ac.jp/record/5755/files/h27ndk346_a.pdf"}, "version_id": "ad7cfaaf-8795-4508-9262-4cede87ed77a"}]}, "item_keyword": {"attribute_name": "\u30ad\u30fc\u30ef\u30fc\u30c9", "attribute_value_mlt": [{"subitem_subject": "DNA methylation", "subitem_subject_scheme": "Other"}, {"subitem_subject": "periodontitis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "rheumatoid arthritis", "subitem_subject_scheme": "Other"}, {"subitem_subject": "tumor necrosis factor-alpha", "subitem_subject_scheme": "Other"}]}, "item_language": {"attribute_name": "\u8a00\u8a9e", "attribute_value_mlt": [{"subitem_language": "eng"}]}, "item_resource_type": {"attribute_name": "\u8cc7\u6e90\u30bf\u30a4\u30d7", "attribute_value_mlt": [{"resourcetype": "thesis", "resourceuri": "http://purl.org/coar/resource_type/c_46ec"}]}, "item_title": "Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis", "item_titles": {"attribute_name": "\u30bf\u30a4\u30c8\u30eb", "attribute_value_mlt": [{"subitem_title": "Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis"}, {"subitem_title": "Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis", "subitem_title_language": "en"}]}, "item_type_id": "6", "owner": "1", "path": ["453/455", "432/433/434"], "permalink_uri": "http://hdl.handle.net/10191/42134", "pubdate": {"attribute_name": "\u516c\u958b\u65e5", "attribute_value": "2018-09-11"}, "publish_date": "2018-09-11", "publish_status": "0", "recid": "5755", "relation": {}, "relation_version_is_last": true, "title": ["Tumor necrosis factor-alpha gene promoter methylation in Japanese adults with chronic periodontitis and rheumatoid arthritis"], "weko_shared_id": 2}