{"created":"2021-03-01T06:09:33.266792+00:00","id":5687,"links":{},"metadata":{"_buckets":{"deposit":"70b6b719-8173-453a-906c-d01ecc923e19"},"_deposit":{"id":"5687","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5687"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005687","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Quantitative analysis of NMDA receptor channel subunits in mouse brain"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2016-03-23","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"20","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2016-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"NMDA型グルタミン酸受容体(NMDAR)は，中枢神経系においてシ ナプス形成やシナプス可塑性など脳機能に関わる分子群である．NMDARは，単一遺伝子にコードされスプライスにより多様性を持つGluN1サブユニットと，4種類のGluN2 サブユニットが2分子ずつ会合して構成されており，それらの組み合わせがチャネル特性を決定する．しかし，脳におけるGluN サブユニットタンパク質の定量的な解析はなく，その構成比率は明らかにされていない．そこで脳各部位におけるNMDARサブユニットを定量するために，AMPA型グルタミン酸受容体サブユニットGluA1を標準物質とした定量的ウエスタンブロットを行った．その結果，大脳皮質，海馬，小脳いずれにおいても GluN1が GluN2より多く存在していたが，NMDARが機能していると考えられているシナプトソーム画分では，ほぼ等量の割合になっていた．また，GluN1のC末端の形状は，C2' 型が C2型よりも多かった，これは小脳でとりわけ顕著で，約 10倍多いと算定された．","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（医学）. 報告番号: 甲第4102号. 学位記番号: 新大院博（医）甲第669号. 学位授与年月日: 平成28年3月23日","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（医）甲第669号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4102号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"50726","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Suzuki, Yasuhiro"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"鈴木, 康浩"}],"nameIdentifiers":[{"nameIdentifier":"50725","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h27nmk669.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/5687/files/h27nmk669.pdf"},"version_id":"cc51472d-3b67-4410-bedc-dad4cf0be753"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h27nmk669_a.pdf","filesize":[{"value":"574.3 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5687/files/h27nmk669_a.pdf"},"version_id":"ad0e04c6-6f1c-4d07-9920-ed1365ff62a9"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"NMDA受容体","subitem_subject_scheme":"Other"},{"subitem_subject":"ウエスタンブロット","subitem_subject_scheme":"Other"},{"subitem_subject":"タンパク質定量","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"NMDA型グルタミン酸受容体の定量的解析．","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"NMDA型グルタミン酸受容体の定量的解析．"},{"subitem_title":"NMDA型グルタミン酸受容体の定量的解析．","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2016-05-19"},"publish_date":"2016-05-19","publish_status":"0","recid":"5687","relation_version_is_last":true,"title":["NMDA型グルタミン酸受容体の定量的解析．"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:38:50.541981+00:00"}