{"created":"2021-03-01T06:09:29.683939+00:00","id":5630,"links":{},"metadata":{"_buckets":{"deposit":"008ead82-87cf-4143-9de7-0edd4456c67f"},"_deposit":{"id":"5630","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5630"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005630","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Liver X receptor-α localizes in nucleolus and stimulates rDNA transcription"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2015-03-23","bibliographicIssueDateType":"Issued"},"bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2015-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"LXRα（Liver X receptor α) はLDL（low density lipoprotein）など酸化ステロールの受容体として機能し、脂質代謝、胆汁酸代謝などに関与する核内受容体である。しかし特異性の高い抗体がこれまでなかったためmRNAレベルの研究が多く、その組織細胞内局在や機能については不明な点が多い。本研究では最近作製されたLXRαに対する特異的抗体を用いて、LXRαの細胞内局在とこれまで知られていなかったリボソーム生合成への関与について解析を行ったので報告する。LXRαは核小体内においてfibrillarin、UBFおよびRNA polymerase I （RNA pol I）の転写活性の高い領域、すなわちdense fibrillar componentと呼ばれる領域に局在することがわかった。HepG2細胞においてRNA pol Iの転写活性をActinomycin D投与によって抑制すると、LXRαは核小体内に局在できず核質へ移行したことからLXRαの核小体内局在はRNA pol Iの転写活性に依存していることがわかった。クロマチン免疫沈降法を用いた検討の結果、LXRαは18S rDNAと28S rDNAに結合することが判明した。さらに、HepG2細胞に対してLXRαを活性化する合成リガンドであるT0901317を投与するとリボゾーム前駆体47S/45S rRNAの転写が促進されたが、LXRαをsiRNAでノックダウンすると転写促進が起こらなかった。以上の結果より、LXRαはリボソーム生合成において重要な役割を担っていることが示唆された。","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（医学）. 報告番号: 乙第2187号. 学位記番号: 新大博（医）乙第1776号. 学位授与年月日: 平成27年3月23日","subitem_description_type":"Other"},{"subitem_description":"新潟医学会雑誌. 2014, 128(9), 447-461.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大博（医）乙第1776号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101乙第2187号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"50626","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Ohashi, Riuko"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"大橋, 瑠子"}],"nameIdentifiers":[{"nameIdentifier":"50625","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h26nmo1776.pdf","filesize":[{"value":"1.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/5630/files/h26nmo1776.pdf"},"version_id":"6204de20-5497-499d-832d-adec90dceb3d"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h26nmo1776_a.pdf","filesize":[{"value":"158.0 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5630/files/h26nmo1776_a.pdf"},"version_id":"62e0fddc-ad69-4c21-a65d-fa870bd71a4a"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"LXRα","subitem_subject_scheme":"Other"},{"subitem_subject":"核小体","subitem_subject_scheme":"Other"},{"subitem_subject":"リボソーム RNA","subitem_subject_scheme":"Other"},{"subitem_subject":"fibrillarin","subitem_subject_scheme":"Other"},{"subitem_subject":"UBF","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"LXRαの核小体への局在とリボソームDNA転写制御","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"LXRαの核小体への局在とリボソームDNA転写制御"},{"subitem_title":"LXRαの核小体への局在とリボソームDNA転写制御","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2015-06-03"},"publish_date":"2015-06-03","publish_status":"0","recid":"5630","relation_version_is_last":true,"title":["LXRαの核小体への局在とリボソームDNA転写制御"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:38:40.910801+00:00"}