{"created":"2021-03-01T06:09:21.704405+00:00","id":5502,"links":{},"metadata":{"_buckets":{"deposit":"8676538c-e0ae-4655-896e-bda7715de617"},"_deposit":{"id":"5502","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5502"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005502","sets":["453:455","468:563:564"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"アサガオにおけるストレス応答花成の遺伝子制御"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"90","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-24"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"貧栄養、低温ストレス処理はアサガオ（Pharbitis nil; synonym Ipomoea nil）品種ムラサキの花成を誘導した。ストレス処理はシロイヌナズナの花成経路統合遺伝子FLOWERING LOCUS T （FT）の2 つのホモログの1 つであるPnFT2 の発現を誘導し、1回の16 時間暗処理はPnFT1 とPnFT2 の両方の発現を誘導した。別の品種テンダンは貧栄養ストレスで花成もPnFT2 発現も誘導されず、低温ストレスでは両者が誘導された。これらのことから、アサガオはストレスによって花成を誘導され、このときPnFT2 の発現が誘導されること、光周的花成とストレス応答花成では異なるPnFT 発現制御がなされていることが示唆された。別の花成経路統合遺伝子SUPPRESSOR OF OVEREXPRESSION OF CO1、FT の上流遺伝子CONSTANS、FCA は花成の有無にかかわらず常に発現し、FLOWERINGLOCUS C、FRIGIDA、FVE の発現は常に見られなかった。また、FT の下流遺伝子ではLEAFY のホモログは常に発現し、FD のホモログの発現は検出されなかった。一方、花成のトリガー遺伝子APETALA1（AP1）のホモログPnAP1 の発現はストレス処理で誘導され、PnFT2 発現、花成反応と正の相関が見られた。PnFT は葉で特異的に発現し、PnAP1 発現は茎頂で認められた。この結果は、葉で発現したPnFT2 が茎頂でPnAP1 の発現を誘導することによって花成が誘導されるという仮説と矛盾しない。PnFT2 をアサガオ、シロイヌナズナで過剰発現させたところ、いずれも早期花成を誘導された。このことから、PnFT2 には花成誘導活性があることが示された。RNA interferenceによるPnFT1 発現抑制は短日処理による光周的花成を完全に抑制したのに対し、PnFT2の発現抑制は光周的花成を部分的にのみ抑制した。このことから、光周的花成では両方のPnFT が発現するものの、PnFT1 が主要な役割を果たしていることが示唆された。ストレス応答花成にはサリチル酸（SA）が関与している。SA 生合成の鍵酵素であるフェニルアラニンアンモニアリアーゼを阻害するアミノオキシ酢酸（AOA）は貧栄養ストレスによっ2て誘導された花成とPnFT2 発現を阻害し、このAOA による阻害効果は外生SA によって回復した。また、外生SA は貧栄養ストレス条件下でPnFT2 発現を促進した。しかし、非ストレス条件下で発現を促進することはなかった。これらの結果は、ストレスによって生合成されたSA がPnFT2 発現を誘導し、それによって花成が誘導されること、しかしSA は必要条件であっても十分条件ではないことを示唆する。他の未知の要因が関与している可能性が考えられる。本研究の結果を総合すると、アサガオのストレス応答花成では、ストレスによって生合成されたSA と他の未知のストレス物質が葉でPnFT2 発現を誘導し、PnFT2 が茎頂でPnAP1 の発現を誘導することによって花成が誘導されるということが示唆された。","subitem_description_type":"Abstract"},{"subitem_description":"Poor-nutrition and low-temperature stress treatments induced the flowering in Pharbitis nil (synonym Ipomoea nil) cv. Violet. The stress treatment induced the expression of PnFT2, one of two homologs of flowering pathway integrator gene FLOWERING LOCUS T (FT) of Arabidopsis thaliana, whereas the single 16-hour dark treatment induced the expression of both PnFT1 and PnFT2. Another cultivar Tendan was not induced flowering and the expression of PnFT2 by the poor-nutrition stress, whereas the low-temperature stress induced both of them. These results suggest that the expression of PnFT2 is induced when P. nil flowered by stress, and PnFT expression is regulated differently between stress-induced and photoperiodic flowering. Another flowering pathway integrator gene, SUPPRESSOR OF OVEREXPRESSION OF CO1 and genes in upstream of FT, CONSTANS and FCA express regardless of flowering status, and the expression of FLOWERING LOCUS C, FRIGIDA and FVE was not detected. Among genes in downstream of FT, LEAFY homolog expressed constitutively and the expression of FD homolog was not detected. The expression of PnAP1, a homolog of APETALA1 (AP1) which is the trigger gene of flowering, was induced by stress treatment, and there was the positive correlation among the expression level of PnFT2 and PnAP1 and intensity of flowering response. PnFT expressed specifically in leaves, and the expression of PnAP1 was observed in shoot apices. This result is consistent with an assumption that PnFT2 induces PnAP1 expression to induce flowering. Overexpression of PnFT2 induces early flowering in P. nil and A. 4 thaliana, indicating that PnFT2 has the activity to induce flowering. The suppression of PnFT1 expression by RNA interference suppressed the photoperiodic flowering completely, whereas the suppression of PnFT2 expression suppressed it only partially. This suggests that both PnFT express in photoperiodic flowering, but PnFT1 plays a main role in it. Salicylic acid (SA) is involved in stress-induced flowering. Aminooxyacetic acid (AOA), the inhibitor of phenylalanine ammonia-lyase, the key enzyme of SA biosynthesis, inhibited the flowering and the PnFT2 expression induced by the poor-nutrition stress, and this inhibition was overcome by the exogenous SA. The exogenous SA enhanced the PnFT2 expression under the poor-nutrition stress conditions, but did not induce it under non-stress conditions. These results suggest that SA of which biosynthesis is induced by stress induces the expression of PnFT2, which induces flowering, and SA is necessary but not sufficient for the PnFT2 expression. It is possible that other unknown factor(s) are involved in stress-induced flowering. Taken together, the present study suggests that SA and other unknown factor which are synthesized by stress induces PnFT2 expression in leaves, PnFT2 induces PnAP1 expression in shoot apices, and then PnAP1 induces flowering in P. nil.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（理学）. 報告番号: 甲第3920号. 学位記番号: 新大院博（理）甲第389号. 学位授与年月日: 平成26年3月24日","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（理）甲第389号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第3920号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"50390","nameIdentifierScheme":"WEKO"}],"names":[{"name":"山田, 瑞樹"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Yamada, Mizuki"}],"nameIdentifiers":[{"nameIdentifier":"50389","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h25fsk389.pdf","filesize":[{"value":"3.3 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/5502/files/h25fsk389.pdf"},"version_id":"0410d71c-cbbc-4748-93d5-2e1d31248aa2"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h25fsk389_a.pdf","filesize":[{"value":"183.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5502/files/h25fsk389_a.pdf"},"version_id":"205209c5-89e3-4ed1-a73b-2fb613bffd32"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Gene regulation of stress-induced flowering in Japanese morning glory","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Gene regulation of stress-induced flowering in Japanese morning glory"},{"subitem_title":"Gene regulation of stress-induced flowering in Japanese morning glory","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","564"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-05-19"},"publish_date":"2014-05-19","publish_status":"0","recid":"5502","relation_version_is_last":true,"title":["Gene regulation of stress-induced flowering in Japanese morning glory"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:38:33.364449+00:00"}