{"created":"2021-03-01T06:09:21.573295+00:00","id":5500,"links":{},"metadata":{"_buckets":{"deposit":"4a686f3b-bef7-4471-babc-93e650187a94"},"_deposit":{"id":"5500","owners":[],"pid":{"revision_id":0,"type":"depid","value":"5500"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00005500","sets":["453:455","468:563:564"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"アフリカツメガエル胚におけるRdd蛋白質の発現と機能の解析"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2014-03-24","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"20","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2014-03-24"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（理学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Previous studies in our laboratory identified Rdd (Repeated D domain-like), a group of novel secretory proteins consisting of repeated domains of cysteine-rich sequence in Xenopus laevis embryo (Shibata et al., 2008). Transcripts of rdd are mainly expressed in the ventral region at the stages from the gastrula to tailbud, and a loss-of-function study indicated that Rdd is involved in the process of blood vessel formation in embryo. In the present study, in order to investigate the biochemical property of Rdd proteins, rabbit anti-serum was raised against a synthesized Rdd peptide. Western blot analysis identified a single band of 28 kD in the tissue extracts from the gastrula to tailbud embryos. In whole-mount immunostaining analysis, positive signal was detected in the regions of intermediate mesoderm, inter-somites, vitelline veins, branchial arches and hypaxial muscle segments at the tailbud stage. In the sections of stained embryo, the signals were observed in the lateral plate mesoderm and the ventral mesoderm. Since the Rdd proteins were also detected in the regions where the neural crest derivatives were located, I examined the expression of neural crest markers, such as sox9 and twist, in the Rdd morpholino (MO) -injected embryo, and found that the expression of these two markers was reduced. Moreover, a lineage-tracing experiment showed that the migration of neural crest cells toward the ventral region was inhibited in the Rdd MO-injected embryo. To investigate whether the localization of Rdd protein in the embryo functionally links to the blood vessel formation, I examined the staining of antibody in the embryos injected with VEGF MO that had been shown to disturb the differentiation of blood vessel. As expected, the staining of anti-Rdd antibody was remarkably reduced by the injection of VEGF MO. Finally, recombinant Rdd protein was traced by an antibody to the FLAG-tag after injection of mRNA in the selected blastomeres of animal pole area, followed by the culture of animal cap explant. The positive signal for detecting rdd protein was found at the inter-cellular space of uninjected cells, in addition to the cytoplasm of injected cells. In a current model represented in this study, it is suggested that secreted Rdd protein interacts with molecule(s) associated with the blood vessel precursor cells and the neural crest cells and is involved in the morphogenesis of these cells.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（理学）. 報告番号: 甲第3918号. 学位記番号: 新大院博（理）甲第387号. 学位授与年月日: 平成26年3月24日","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博（理）甲第387号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第3918号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"50386","nameIdentifierScheme":"WEKO"}],"names":[{"name":"林, 鍾賛"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Lim, Jong-Chan"}],"nameIdentifiers":[{"nameIdentifier":"50385","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h25fsk387.pdf","filesize":[{"value":"2.1 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/5500/files/h25fsk387.pdf"},"version_id":"ccc8a21d-da32-4e57-aa2b-ec74a43438da"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"h25fsk387_a.pdf","filesize":[{"value":"183.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/5500/files/h25fsk387_a.pdf"},"version_id":"5f513a4b-9896-45f4-aede-e8547b5c1966"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Studies on expression and function of Rdd, a novel secretory protein, in Xenopus embryo","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Studies on expression and function of Rdd, a novel secretory protein, in Xenopus embryo"},{"subitem_title":"Studies on expression and function of Rdd, a novel secretory protein, in Xenopus embryo","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","564"],"pubdate":{"attribute_name":"公開日","attribute_value":"2014-05-19"},"publish_date":"2014-05-19","publish_status":"0","recid":"5500","relation_version_is_last":true,"title":["Studies on expression and function of Rdd, a novel secretory protein, in Xenopus embryo"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:38:30.801213+00:00"}