@misc{oai:niigata-u.repo.nii.ac.jp:00005267,
 author = {小林, 寿美},
 month = {Mar},
 note = {The growth and multiplication of clostridia are undesirable in silage fermentation, as they produce butyric acid and degrade protein and amino acids to ammonia. The species of clostridia that is related to silage fermentation has not been clarified the factors that affect the fermentation quality. The present study was aimed to determine the taxonomy and physiology of clostridia isolated from silage and the effects of lactic acid bacteria and the chemical composition of silage material on microbial population and fermentation quality. 1. The lactic acid bacteria inoculants and the moisture contents of silage material which affect the fermentation quality and microorganism composition were studied. Silage inoculated with Lactobacillus plantarum and low-moisture silage had higher lactic acid contents but lower butyric acid contents compared to the control silage. Lactobasillus plantarum and low-moisture contents of silage material were also found to inhibit butyric fermentation without significantly different clostridial counts among treatments. Therefore, these results suggest that they affect the species structure of the clostridial population. 2. The phenotypic and genotypic characterizations of clostridia isolated from silage were studied. A total of 78 clostridia isolated from Italian ryegrass, alfalfa, mixed pasture of timothy and orchard grass and silages prepared by these grasses. According to biochemical characteristics and 16S rRNA gene sequence analysis, 61 isolates were identified as C. aerotorelans, C. algidixylanolyticum, C beijerinckii, C. bifermentans, C. cerelecrescens, C. diolis, C. frigicarnis, C. hydrogeniformans, C. perfringens C. roseum, C. sartagoforme, C. scatologenes, C. tunisiense and C. xylanolyticum. Analysis of the 16S rRNA gene sequence of the eight isolates of 78 previously isolates showed that they were closely related to C. sporogenes and C. botulinum, one isolate was closely related to C. sulfidigenes and C. thiosulfatireducens. Eight isolates were suggested to be new species of clostridia., 新潟大学大学院自然科学研究科, 平成23年3月23日, 新大院博（農）甲第111号, 新大院博（農）甲第111号},
 title = {サイレージ発酵品質に影響を及ぼす要因の解析およびサイレージ発酵に関与するClostridiaの分類学的研究},
 year = {2011}
}