{"created":"2021-03-01T06:08:13.382146+00:00","id":4423,"links":{},"metadata":{"_buckets":{"deposit":"c9a5d296-a86f-4f7d-83aa-eaf4a2bb4c75"},"_deposit":{"id":"4423","owners":[],"pid":{"revision_id":0,"type":"depid","value":"4423"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00004423","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"In Vitro Sensitization(IVS) 培養法による,担癌宿主からの抗腫瘍エフェクターT細胞の誘導 : エフェクター前駆細胞,抗原腫瘍細胞とエフェクターT細胞自身の凍結保存性および,エフェクターT細胞の長期培養に関する研究"}]},"item_6_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"1994-11-08","bibliographicIssueDateType":"Issued"},"bibliographicPageEnd":"13","bibliographicPageStart":"1","bibliographic_titles":[{}]}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"1994-11-08"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士（医学）"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"Using weakly immunogenic murine MCA 105 and 106 fibrosarcomas, we have previously demonstrated that therapeutic cells could be generated by an in vitro sensitization (IVS) culture with which lymphocytes from tumor-bearing mice were co-cultured with viable autologous tumor cells in the presence of low dose of recombinant interleukin-2 (RIL-2). Adoptive transfer of the IVS cells could effectively mediate not only the regression of established visceral metastasis, but also cure and result in long term immune to autologous tumor challenge in treated mice. Applying this IVS culture system clinically, it would be ideal to control the initiation of the IVS culture, especially when combining with other treatment, such as chemotherapy and irradiation. In the present study, feasibility of cryopreservation of responder lymphocytes, stimulator tumor cells before IVS culture, and of the effector IVS cells were evaluated. The data presented here demonstrated that responder lymphoid cells obtained from tumor-bearing mice could be successfully cryopreserved while maintaining their ability to be sensitized. Fresh tumor cells, as well as established tumor cell lines in vitro could be cryopreserved without losing antigenic stimulator potential during IVS culture. Furthermore, the IVS cells generated in the initial 9-day IVS culture could be expanded while maintaining their anti-tumor effect in vivo by periodic antigenic stimulation with fresh or in vitro cultured tumor cells in the presence of RIL-2. Our data suggested that IVS culture could be initiated at optimal time which enables us to combine the adoptive immunotherapy with other therapy in various clinical situations.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"学位の種類: 博士（医学）. 報告番号: 乙第1437号. 学位記番号: 新大博（医）乙第1306号. 学位授与年月日: 平成6年11月8日","subitem_description_type":"Other"},{"subitem_description":"日本癌治療学会誌. 1994, 29(5), 733-745.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大博（医）乙第1306号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101B1437"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"48420","nameIdentifierScheme":"WEKO"}],"names":[{"name":"若林, 昌哉"}]}]},"item_6_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟大学"}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Wakabayashi, Masaya"}],"nameIdentifiers":[{"nameIdentifier":"48419","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2019-08-05"}],"displaytype":"detail","filename":"000000280134.pdf","filesize":[{"value":"1.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/4423/files/000000280134.pdf"},"version_id":"7d60cd52-bae4-4944-83b3-3cc471da2071"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Adoptive immunotherapy","subitem_subject_scheme":"Other"},{"subitem_subject":"In vitro sensitization","subitem_subject_scheme":"Other"},{"subitem_subject":"Cryopreservation","subitem_subject_scheme":"Other"},{"subitem_subject":"Long term expansion of effector cells","subitem_subject_scheme":"Other"},{"subitem_subject":"Pulmonary metastasis","subitem_subject_scheme":"Other"},{"subitem_subject":"養子免疫療法","subitem_subject_scheme":"Other"},{"subitem_subject":"凍結保存","subitem_subject_scheme":"Other"},{"subitem_subject":"肺転移","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Generation of Therapeutic T Lymphocytes from Tumor-bearing Mice by the in vitro Sensitization(IVS) : Feasibility of Cryopreservation of Preeffector Cells, Effector IVS Cells and Long Term Expansion of Effector Cells","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Generation of Therapeutic T Lymphocytes from Tumor-bearing Mice by the in vitro Sensitization(IVS) : Feasibility of Cryopreservation of Preeffector Cells, Effector IVS Cells and Long Term Expansion of Effector Cells"},{"subitem_title":"Generation of Therapeutic T Lymphocytes from Tumor-bearing Mice by the in vitro Sensitization(IVS) : Feasibility of Cryopreservation of Preeffector Cells, Effector IVS Cells and Long Term Expansion of Effector Cells","subitem_title_language":"en"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2013-10-10"},"publish_date":"2013-10-10","publish_status":"0","recid":"4423","relation_version_is_last":true,"title":["Generation of Therapeutic T Lymphocytes from Tumor-bearing Mice by the in vitro Sensitization(IVS) : Feasibility of Cryopreservation of Preeffector Cells, Effector IVS Cells and Long Term Expansion of Effector Cells"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T03:37:14.419374+00:00"}