@article{oai:niigata-u.repo.nii.ac.jp:00004042,
 author = {Ishiyama, Yohei and Takata, Takeomi and Nakanishi, Toshihiro and Kaneoke, Mitsuoki and Watanabe, Ken-ichi and Yanagida, Fujitoshi and Chen, Yi-sheng and Kouya, Tomoaki and Tanaka, Takaaki and Taniguchi, Masayuki},
 issue = {4},
 journal = {日本食品工学会誌},
 month = {Dec},
 note = {We investigated the antimicrobial activity of several bacteriocins, which were produced by newly　isolated lactic acid bacteria in koji extract medium supplemented with rice protein hydrolyzate (RPH),　against strains of NBRC type culture and spoilage bacteria isolated from deteriorated sake. The partial　sequences of the 16S rRNA gene of the spoilage bacteria isolated from sake showed a high similarity　to those of Lactobacillus fructivorans, Lactobacillus hilgardii, and Lactobacillus paracasei, with　99.6-100% identity. Enterococcus durans C102901 (C102901), Lactococcus lactis subsp. lactis C101910　(C101910), and Lactococcus lactis subsp. lactis NBRC 12007 (NBRC 12007) grew well and produced　bacteriocins with high activity in koji extract medium supplemented with RPH. When culture　supernatants containing bacteriocins from C101910 and NBRC 12007 were added to the medium at a　volume ratio of 10% (v/v), the growth of L. fructivorans NBRC 13954T was significantly inhibited and　the viable cell concentration decreased below the detection limit (1.0•~102 cells/ml) at 4 h. Further,　by the addition of bacteriocin solutions from C102901, C101910, and NBRC 12007 to the medium at　a volume ratio of 1% (v/v), the growth of L. hilgardii NBRC 15886T and H130 (closely related to L.　hilgardii) isolated from putrid sake was bactericidally inhibited and the colony-forming units fell by　more than three orders of magnitude within 4-12 h as compared with the initial cell concentration., 我々は米タンパク質加水分解物(RPH)を添加した麹汁培地を用いて,新奇に単離した乳酸菌によるバクテリオシン生産について検討した.また,生産されたバクテリオシンの清酒の腐敗に関連する細菌(火落菌)に対する抗菌活性についても検討した.清酒より単離した火落菌の16S rRNA遺伝子の部分塩基配列は,Lactobacillus fructivorans, Lactobacillus hilgardiiおよびLactobacillus payacaseiと高い相同性を示した.バクテリオシン生産菌Enteyococcus durans C102901(C102901),Lactococcus lactis subsp.lactis C101910(C101910)およびLactococcus lactis subsp.lactisNBRC 12007(NBRC 12007)はRPHを添加した麹汁培地中で良好な増殖を示し,高活性なバクテリオシンを生産した.C101910とNBRC 12007によって生産されたバクテリオシン溶液を培地に対して10%(v/v)の割合で添加した結果,L.fructivorans NBRC 13954Tの増殖は顕著に阻害され,その生菌数は,4時間目には検出限界(1.0×102cells/ml)以下まで減少した.また,C102901,C101910およびNBRC12007によって生産されたバクテリオシン溶液を培地に対して1%(v/v)の割合で添加した結果,L.hilgardii NBRC 15886TとH130株(単離した菌株)の増殖は殺菌的に抑制され,その生菌数は,初期の生菌数と比較して,培養4時間から12時間以内に3オーダー以上減少した.},
 pages = {277--286},
 title = {Production of Bacteriocins by Several Lactic Acid Bacteria and Their Application to Growth Inhibition of Spoilage Bacteria Related to Hiochi},
 volume = {9},
 year = {2008}
}