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  1. 060 工学部
  2. 10 学術雑誌論文
  3. 10 査読済論文
  1. 0 資料タイプ別
  2. 01 学術雑誌論文

Production of Bacteriocins by Several Lactic Acid Bacteria and Their Application to Growth Inhibition of Spoilage Bacteria Related to Hiochi

http://hdl.handle.net/10191/26346
http://hdl.handle.net/10191/26346
c95d6527-af6a-48fa-bb91-81c6aa227f6e
名前 / ファイル ライセンス アクション
9_4_277-286.pdf 9_4_277-286.pdf (1.6 MB)
Item type 学術雑誌論文 / Journal Article(1)
公開日 2014-03-17
タイトル
言語 en
タイトル Production of Bacteriocins by Several Lactic Acid Bacteria and Their Application to Growth Inhibition of Spoilage Bacteria Related to Hiochi
言語
言語 eng
キーワード
主題Scheme Other
主題 bacteriocin
キーワード
主題Scheme Other
主題 hiochi bacteria
キーワード
主題Scheme Other
主題 Lactobacillus
キーワード
主題Scheme Other
主題 koji extract medium
キーワード
主題Scheme Other
主題 rice protein
資源タイプ
資源 http://purl.org/coar/resource_type/c_6501
タイプ journal article
その他のタイトル
その他のタイトル 乳酸菌によるバクテリオシンの生産とそれらの火落ち関連腐敗細菌の増殖抑制への応用
言語 ja
著者 Ishiyama, Yohei

× Ishiyama, Yohei

WEKO 47026

en Ishiyama, Yohei

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Takata, Takeomi

× Takata, Takeomi

WEKO 47027

en Takata, Takeomi

Search repository
Nakanishi, Toshihiro

× Nakanishi, Toshihiro

WEKO 47028

en Nakanishi, Toshihiro

Search repository
Kaneoke, Mitsuoki

× Kaneoke, Mitsuoki

WEKO 47029

en Kaneoke, Mitsuoki

Search repository
Watanabe, Ken-ichi

× Watanabe, Ken-ichi

WEKO 47030

en Watanabe, Ken-ichi

Search repository
Yanagida, Fujitoshi

× Yanagida, Fujitoshi

WEKO 47031

en Yanagida, Fujitoshi

Search repository
Chen, Yi-sheng

× Chen, Yi-sheng

WEKO 47032

en Chen, Yi-sheng

Search repository
Kouya, Tomoaki

× Kouya, Tomoaki

WEKO 47033

en Kouya, Tomoaki

Search repository
Tanaka, Takaaki

× Tanaka, Takaaki

WEKO 47034

en Tanaka, Takaaki

Search repository
Taniguchi, Masayuki

× Taniguchi, Masayuki

WEKO 47035

en Taniguchi, Masayuki

Search repository
著者別名
識別子 47036
識別子Scheme WEKO
姓名 石山, 洋平
言語 ja
著者別名
識別子 47037
識別子Scheme WEKO
姓名 高田, 剛臣
言語 ja
著者別名
識別子 47038
識別子Scheme WEKO
姓名 中西, 利公
言語 en
著者別名
識別子 47039
識別子Scheme WEKO
姓名 金桶, 光起
言語 ja
著者別名
識別子 47040
識別子Scheme WEKO
姓名 渡邊, 健一
言語 ja
著者別名
識別子 47041
識別子Scheme WEKO
姓名 柳田, 藤寿
言語 en
著者別名
識別子 47042
識別子Scheme WEKO
姓名 陳, 奕伸
言語 en
著者別名
識別子 47043
識別子Scheme WEKO
姓名 高屋, 朋彰
言語 en
著者別名
識別子 47044
識別子Scheme WEKO
姓名 田中, 孝明
言語 en
著者別名
識別子 47045
識別子Scheme WEKO
姓名 谷口, 正之
言語 ja
抄録
内容記述タイプ Abstract
内容記述 We investigated the antimicrobial activity of several bacteriocins, which were produced by newly isolated lactic acid bacteria in koji extract medium supplemented with rice protein hydrolyzate (RPH), against strains of NBRC type culture and spoilage bacteria isolated from deteriorated sake. The partial sequences of the 16S rRNA gene of the spoilage bacteria isolated from sake showed a high similarity to those of Lactobacillus fructivorans, Lactobacillus hilgardii, and Lactobacillus paracasei, with 99.6-100% identity. Enterococcus durans C102901 (C102901), Lactococcus lactis subsp. lactis C101910 (C101910), and Lactococcus lactis subsp. lactis NBRC 12007 (NBRC 12007) grew well and produced bacteriocins with high activity in koji extract medium supplemented with RPH. When culture supernatants containing bacteriocins from C101910 and NBRC 12007 were added to the medium at a volume ratio of 10% (v/v), the growth of L. fructivorans NBRC 13954T was significantly inhibited and the viable cell concentration decreased below the detection limit (1.0•~102 cells/ml) at 4 h. Further, by the addition of bacteriocin solutions from C102901, C101910, and NBRC 12007 to the medium at a volume ratio of 1% (v/v), the growth of L. hilgardii NBRC 15886T and H130 (closely related to L. hilgardii) isolated from putrid sake was bactericidally inhibited and the colony-forming units fell by more than three orders of magnitude within 4-12 h as compared with the initial cell concentration.
言語 en
抄録
内容記述タイプ Abstract
内容記述 我々は米タンパク質加水分解物(RPH)を添加した麹汁培地を用いて,新奇に単離した乳酸菌によるバクテリオシン生産について検討した.また,生産されたバクテリオシンの清酒の腐敗に関連する細菌(火落菌)に対する抗菌活性についても検討した.清酒より単離した火落菌の16S rRNA遺伝子の部分塩基配列は,Lactobacillus fructivorans, Lactobacillus hilgardiiおよびLactobacillus payacaseiと高い相同性を示した.バクテリオシン生産菌Enteyococcus durans C102901(C102901),Lactococcus lactis subsp.lactis C101910(C101910)およびLactococcus lactis subsp.lactisNBRC 12007(NBRC 12007)はRPHを添加した麹汁培地中で良好な増殖を示し,高活性なバクテリオシンを生産した.C101910とNBRC 12007によって生産されたバクテリオシン溶液を培地に対して10%(v/v)の割合で添加した結果,L.fructivorans NBRC 13954Tの増殖は顕著に阻害され,その生菌数は,4時間目には検出限界(1.0×102cells/ml)以下まで減少した.また,C102901,C101910およびNBRC12007によって生産されたバクテリオシン溶液を培地に対して1%(v/v)の割合で添加した結果,L.hilgardii NBRC 15886TとH130株(単離した菌株)の増殖は殺菌的に抑制され,その生菌数は,初期の生菌数と比較して,培養4時間から12時間以内に3オーダー以上減少した.
言語 ja
書誌情報 ja : 日本食品工学会誌

巻 9, 号 4, p. 277-286, 発行日 2008-12
出版者
言語 ja
出版者 日本食品工学会
ISSN
収録物識別子タイプ ISSN
収録物識別子 1345-7942
書誌レコードID
収録物識別子タイプ NCID
収録物識別子 AA12076107
DOI
識別子タイプ DOI
関連識別子 http://doi.org/10.11301/jsfe2000.9.277
権利
権利情報 Copyright (C) 2008 Japan Society for Food Engineering
権利
権利情報 本文データは学協会の許諾に基づきJ-Stageから複製したものである
出版タイプ
出版タイプ VoR
出版タイプResource http://purl.org/coar/version/c_970fb48d4fbd8a85
著者版フラグ
値 publisher
異版である
関連タイプ isVersionOf
識別子タイプ URI
関連識別子 https://www.jstage.jst.go.jp/article/jsfe2000/9/4/9_4_277/_pdf
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