{"created":"2021-03-01T06:40:31.617372+00:00","id":34073,"links":{},"metadata":{"_buckets":{"deposit":"9f2bdf36-02a5-4100-a283-de0b9feceafb"},"_deposit":{"id":"34073","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34073"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00034073","sets":["453:456","471:537:538:2011"]},"item_7_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"Monitoring of Mitophagy using mito-Keima system"}]},"item_7_biblio_info_6":{"attribute_name":"書誌情報","attribute_value_mlt":[{"bibliographicIssueDates":{"bibliographicIssueDate":"2019-10","bibliographicIssueDateType":"Issued"},"bibliographicIssueNumber":"9-10","bibliographicPageEnd":"327","bibliographicPageStart":"319","bibliographicVolumeNumber":"133","bibliographic_titles":[{"bibliographic_title":"新潟医学会雑誌"}]}]},"item_7_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"マイトファジーは，不要となったミトコンドリアをオートファジーを介して分解除去することで，ミトコンドリアの恒常性維持に寄与している．これまでの研究から，マイトファジーにはParkin依存的経路とParkin非依存的経路が知られているが，簡便で高感度の検出法はなかった．近年pH感受性蛍光タンパク質Keimaを用いたマイトファジー検出システム(mito-Keima)が報告された．本研究では，mito-KeimaシステムのParkin依存的経路・非依存的経路それぞれに対する感受性・特異性について，mito-Keima安定発現細胞株を用いて検討した．その結果，mito-Keima細胞株を用いた検出システムは，従来法に比べ高感度であり，特異性も高いことが明らかとなった．","subitem_description_type":"Abstract"}]},"item_7_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"177884","nameIdentifierScheme":"WEKO"}],"names":[{"name":"Chernyshova, Kseniia"}]}]},"item_7_publisher_7":{"attribute_name":"出版者","attribute_value_mlt":[{"subitem_publisher":"新潟医学会"}]},"item_7_relation_31":{"attribute_name":"異版である","attribute_value_mlt":[{"subitem_relation_type":"isVersionOf","subitem_relation_type_id":{"subitem_relation_type_id_text":"http://hdl.handle.net/10191/51718","subitem_relation_type_select":"URI"}}]},"item_7_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"publisher"}]},"item_7_source_id_11":{"attribute_name":"書誌レコードID","attribute_value_mlt":[{"subitem_source_identifier":"AN00182415","subitem_source_identifier_type":"NCID"}]},"item_7_source_id_9":{"attribute_name":"ISSN","attribute_value_mlt":[{"subitem_source_identifier":"00290440","subitem_source_identifier_type":"ISSN"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"チェルヌショワ, クセニャ"}],"nameIdentifiers":[{"nameIdentifier":"178132","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-09-15"}],"displaytype":"detail","filename":"133(9-10)_319-327.pdf","filesize":[{"value":"5.2 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"133(9-10)_319-327","url":"https://niigata-u.repo.nii.ac.jp/record/34073/files/133(9-10)_319-327.pdf"},"version_id":"52a26f17-3301-4739-8d68-f5844bf9ba67"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"マイトファジー","subitem_subject_scheme":"Other"},{"subitem_subject":"Parkin","subitem_subject_scheme":"Other"},{"subitem_subject":"mito-Keima","subitem_subject_scheme":"Other"},{"subitem_subject":"ミトコンドリア","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"jpn"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"departmental bulletin paper","resourceuri":"http://purl.org/coar/resource_type/c_6501"}]},"item_title":"mito-Keimaを用いたマイトファジーモニタリングシステムの検討","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"mito-Keimaを用いたマイトファジーモニタリングシステムの検討"}]},"item_type_id":"7","owner":"1","path":["456","2011"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-09-15"},"publish_date":"2020-09-15","publish_status":"0","recid":"34073","relation_version_is_last":true,"title":["mito-Keimaを用いたマイトファジーモニタリングシステムの検討"],"weko_creator_id":"1","weko_shared_id":2},"updated":"2022-12-15T04:04:25.670531+00:00"}