{"created":"2021-03-01T06:40:29.113589+00:00","id":34036,"links":{},"metadata":{"_buckets":{"deposit":"d50b2a0e-fb09-47ea-b849-60b0bb24a6ab"},"_deposit":{"id":"34036","owners":[],"pid":{"revision_id":0,"type":"depid","value":"34036"},"status":"published"},"_oai":{"id":"oai:niigata-u.repo.nii.ac.jp:00034036","sets":["453:455","471:561:562"]},"item_6_alternative_title_1":{"attribute_name":"その他のタイトル","attribute_value_mlt":[{"subitem_alternative_title":"軸索伸長・再生に関与する微小管重合促進タンパク質MAP 1Bのリン酸化部位"}]},"item_6_date_granted_51":{"attribute_name":"学位授与年月日","attribute_value_mlt":[{"subitem_dategranted":"2020-03-23"}]},"item_6_degree_grantor_49":{"attribute_name":"学位授与機関","attribute_value_mlt":[{"subitem_degreegrantor":[{"subitem_degreegrantor_name":"新潟大学"}]}]},"item_6_degree_name_48":{"attribute_name":"学位名","attribute_value_mlt":[{"subitem_degreename":"博士(医学)"}]},"item_6_description_4":{"attribute_name":"抄録","attribute_value_mlt":[{"subitem_description":"The growth cone is a specialized structure that forms at the tip of extending axons in developing and regenerating neurons. This structure is essential for accurate synaptogenesis at developmental stages, and is also involved in plasticity-dependent synaptogenesis and axon regeneration in the mature brain. Thus, understanding the molecular mechanisms utilized by growth cones is indispensable to understanding neuronal network formation and rearrangement. Phosphorylation is the most important and commonly utilized protein modification in signal transduction. We previously identified microtubule-associated protein 1B (MAP 1B) as the most frequently phosphorylated protein among ~ 1200 phosphorylated proteins. MAP 1B has more than 10 phosphorylation sites that were present more than 50 times among these 1200 proteins. Here, we produced phospho-specific antibodies against phosphorylated serines at positions 25 and 1201 of MAP 1B that specifically recognize growing axons both in cultured neurons and in vivo in various regions of the embryonic brain. Following sciatic nerve injury, immunoreactivity with each antibody increased compared to the sham operated group. Experiments with transected and sutured nerves revealed that regenerating axons were specifically recognized by these antibodies. These results suggest that these MAP 1B phosphorylation sites are specifically involved in axon growth and that phospho-specific antibodies against MAP 1B are useful markers of growing/regenerating axons.","subitem_description_type":"Abstract"}]},"item_6_description_5":{"attribute_name":"内容記述","attribute_value_mlt":[{"subitem_description":"Molecular Brain. 2019, 12, 93.","subitem_description_type":"Other"}]},"item_6_description_53":{"attribute_name":"学位記番号","attribute_value_mlt":[{"subitem_description":"新大院博(医)甲第945号","subitem_description_type":"Other"}]},"item_6_dissertation_number_52":{"attribute_name":"学位授与番号","attribute_value_mlt":[{"subitem_dissertationnumber":"13101甲第4711号"}]},"item_6_full_name_3":{"attribute_name":"著者別名","attribute_value_mlt":[{"nameIdentifiers":[{"nameIdentifier":"178071","nameIdentifierScheme":"WEKO"}],"names":[{"name":"石川, 裕也"}]}]},"item_6_relation_14":{"attribute_name":"DOI","attribute_value_mlt":[{"subitem_relation_type_id":{"subitem_relation_type_id_text":"info:doi/10.1186/s13041-019-0510-z","subitem_relation_type_select":"DOI"}}]},"item_6_select_19":{"attribute_name":"著者版フラグ","attribute_value_mlt":[{"subitem_select_item":"ETD"}]},"item_creator":{"attribute_name":"著者","attribute_type":"creator","attribute_value_mlt":[{"creatorNames":[{"creatorName":"Ishikawa, Yuya"}],"nameIdentifiers":[{"nameIdentifier":"178070","nameIdentifierScheme":"WEKO"}]}]},"item_files":{"attribute_name":"ファイル情報","attribute_type":"file","attribute_value_mlt":[{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-08-31"}],"displaytype":"detail","filename":"r1nmk945.pdf","filesize":[{"value":"6.0 MB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"本文","url":"https://niigata-u.repo.nii.ac.jp/record/34036/files/r1nmk945.pdf"},"version_id":"369d0949-22d5-44b5-afbb-5e557910f964"},{"accessrole":"open_date","date":[{"dateType":"Available","dateValue":"2020-08-31"}],"displaytype":"detail","filename":"r1nmk945_a.pdf","filesize":[{"value":"570.8 kB"}],"format":"application/pdf","licensetype":"license_note","mimetype":"application/pdf","url":{"label":"要旨","url":"https://niigata-u.repo.nii.ac.jp/record/34036/files/r1nmk945_a.pdf"},"version_id":"6fb72a2a-431f-4ab2-8955-4df92fee87df"}]},"item_keyword":{"attribute_name":"キーワード","attribute_value_mlt":[{"subitem_subject":"Phosphorylation","subitem_subject_scheme":"Other"},{"subitem_subject":"MAP 1B","subitem_subject_scheme":"Other"},{"subitem_subject":"Development","subitem_subject_scheme":"Other"},{"subitem_subject":"Growth cone","subitem_subject_scheme":"Other"},{"subitem_subject":"Axon regeneration","subitem_subject_scheme":"Other"}]},"item_language":{"attribute_name":"言語","attribute_value_mlt":[{"subitem_language":"eng"}]},"item_resource_type":{"attribute_name":"資源タイプ","attribute_value_mlt":[{"resourcetype":"thesis","resourceuri":"http://purl.org/coar/resource_type/c_46ec"}]},"item_title":"Phosphorylation sites of microtubuleassociated protein 1B (MAP 1B) are involved in axon growth and regeneration","item_titles":{"attribute_name":"タイトル","attribute_value_mlt":[{"subitem_title":"Phosphorylation sites of microtubuleassociated protein 1B (MAP 1B) are involved in axon growth and regeneration"}]},"item_type_id":"6","owner":"1","path":["455","562"],"pubdate":{"attribute_name":"公開日","attribute_value":"2020-08-31"},"publish_date":"2020-08-31","publish_status":"0","recid":"34036","relation_version_is_last":true,"title":["Phosphorylation sites of microtubuleassociated protein 1B (MAP 1B) are involved in axon growth and regeneration"],"weko_creator_id":"1","weko_shared_id":null},"updated":"2022-12-15T04:04:18.865713+00:00"}